AUTHOR=Jouhten Hanne , Ronkainen Aki , Aakko Juhani , Salminen Seppo , Mattila Eero , Arkkila Perttu , Satokari Reetta
TITLE=Cultivation and Genomics Prove Long-Term Colonization of Donor’s Bifidobacteria in Recurrent Clostridioides difficile Patients Treated With Fecal Microbiota Transplantation
JOURNAL=Frontiers in Microbiology
VOLUME=11
YEAR=2020
URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01663
DOI=10.3389/fmicb.2020.01663
ISSN=1664-302X
ABSTRACT=
Fecal microbiota transplantation (FMT) is an effective treatment for recurrent Clostridioides difficile infection (rCDI) and it’s also considered for treating other indications. Metagenomic studies have indicated that commensal donor bacteria may colonize FMT recipients, but cultivation has not been employed to verify strain-level colonization. We combined molecular profiling of Bifidobacterium populations with cultivation, molecular typing, and whole genome sequencing (WGS) to isolate and identify strains that were transferred from donors to recipients. Several Bifidobacterium strains from two donors were recovered from 13 recipients during the 1-year follow-up period after FMT. The strain identities were confirmed by WGS and comparative genomics. Our results show that specific donor-derived bifidobacteria can colonize rCDI patients for at least 1 year, and thus FMT may have long-term consequences for the recipient‘s microbiota and health. Conceptually, we demonstrate that FMT trials combined with microbial profiling can be used as a platform for discovering and isolating commensal strains with proven colonization capacity for potential therapeutic use.