AUTHOR=Huang Mingzhu , Zhao Yue , Feng Lin , Zhu Lingfeng , Zhan Li , Chen Xuelan 

TITLE=Role of ClpB From Corynebacterium crenatum in Thermal Stress and Arginine Fermentation

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01660

DOI=10.3389/fmicb.2020.01660

ISSN=1664-302X

ABSTRACT=<p>ClpB, an ATP-dependent molecular chaperone, is involved in metabolic pathways and plays important roles in microorganisms under stress conditions. Metabolic pathways and stress resistance are important characteristics of industrially -relevant bacteria during fermentation. Nevertheless, ClpB-related observations have been rarely reported in industrially -relevant microorganisms. Herein, we found a homolog of ClpB from <italic>Corynebacterium crenatum</italic>. The amino acid sequence of ClpB was analyzed, and the recombinant ClpB protein was purified and characterized. The full function of ClpB requires DnaK as chaperone protein. For this reason, <italic>dnaK</italic>/<italic>clpB</italic> deletion mutants and the complemented strains were constructed to investigate the role of ClpB. The results showed that DnaK/ClpB is not essential for the survival of <italic>C. crenatum</italic> MT under pH and alcohol stresses. The ClpB-deficient or DnaK-deficient <italic>C. crenatum</italic> mutants showed weakened growth during thermal stress. In addition, the results demonstrated that deletion of the <italic>clpB</italic> gene affected glucose consumption and <sc>L</sc>-arginine, <sc>L</sc>-glutamate, and lactate production during fermentation.</p>