AUTHOR=Lv Binna , Jiang Na , Hasan Rakibul , Chen Yingying , Sun Manhong , Li Shidong 

TITLE=Cell Wall Biogenesis Protein Phosphatase CrSsd1 Is Required for Conidiation, Cell Wall Integrity, and Mycoparasitism in Clonostachys rosea

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01640

DOI=10.3389/fmicb.2020.01640

ISSN=1664-302X

ABSTRACT=<p>Cell wall biogenesis protein phosphatases play important roles in various cellular processes in fungi. However, their functions in the widely distributed mycoparasitic fungus <italic>Clonostachys rosea</italic> remain unclear, as do their potential for controlling plant fungal diseases. Herein, the function of cell wall biogenesis protein phosphatase CrSsd1 in <italic>C. rosea</italic> 67-1 was investigated using gene disruption and complementation approaches. The gene-deficient mutant ΔCrSsd1 exhibited much lower conidiation, hyphal growth, mycoparasitic ability, and biocontrol efficacy than the wild-type (WT) strain, and it was more sensitive to sorbitol and Congo red. The results indicate that <italic>CrSsd1</italic> is involved in fungal conidiation, osmotic stress adaptation, cell wall integrity, and mycoparasitism in <italic>C. rosea</italic>.</p>