AUTHOR=Yao Hong , Cheng Jing , Li Aijuan , Yu Runhao , Zhao Wenbo , Qin Shangshang , Du Xiang-Dang 

TITLE=Molecular Characterization of an IncFIIk Plasmid Co-harboring blaIMP–26 and tet(A) Variant in a Clinical Klebsiella pneumoniae Isolate

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01610

DOI=10.3389/fmicb.2020.01610

ISSN=1664-302X

ABSTRACT=<p>Carbapenems and tigecycline are two important classes of antimicrobial agents to treat the infections caused by Enterobacterales. Here, we reported a plasmid carrying both <italic>bla</italic><sub>IMP–26</sub> and <italic>tet</italic>(A) variant in clinical <italic>Klebsiella pneumoniae</italic> KP-1572. MIC results showed that <italic>K. pneumonia</italic> KP-1572 was resistant to a wide range of antimicrobials. The <italic>bla</italic><sub>IMP–26</sub> and <italic>tet</italic>(A) variant were located on an identical plasmid, which was indicated by S1-PFGE and southern blotting hybridization and can be successfully transferred by electroporation. Whole-plasmid sequencing and analysis revealed that a 142,993-bp-sized plasmid, designated pIMP1572, contains an IncFII<sub>k</sub> backbone and a variable region harboring <italic>bla</italic><sub>IMP–26</sub> and <italic>tet</italic>(A) variant. The plasmid pIMP1572 was apparently originated from a <italic>tet</italic>(A)-carrying IncFII<sub>k</sub> plasmid but with a deletion length of 6,216-bp and a multiple drug resistance region (MDRR) insertion of 25,259 bp. The plasmid pIMP1572 in the present study represents the first report of the IncFII<sub>k</sub> plasmid co-carrying <italic>bla</italic><sub>IMP</sub> and <italic>tet</italic>(A) variant, which should be monitored.</p>