AUTHOR=Hu Dini , Sun Chenghang , Jin Tao , Fan Guangyi , Mok Kai Meng , Li Kai , Lee Simon Ming-Yuen 

TITLE=Exploring the Potential of Antibiotic Production From Rare Actinobacteria by Whole-Genome Sequencing and Guided MS/MS Analysis

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01540

DOI=10.3389/fmicb.2020.01540

ISSN=1664-302X

ABSTRACT=<p>Actinobacteria are well recognized for their production of structurally diverse bioactive secondary metabolites, but the rare actinobacterial genera have been underexploited for such potential. To search for new sources of active compounds, an experiment combining genomic analysis and tandem mass spectrometry (MS/MS) screening was designed to isolate and characterize actinobacterial strains from a mangrove environment in Macau. Fourteen actinobacterial strains were isolated from the collected samples. Partial 16S sequences indicated that they were from six genera, including <italic>Brevibacterium</italic>, <italic>Curtobacterium</italic>, <italic>Kineococcus</italic>, <italic>Micromonospora</italic>, <italic>Mycobacterium</italic>, and <italic>Streptomyces</italic>. The isolate sp.01 showing 99.28% sequence similarity with a reference rare actinobacterial species <italic>Micromonospora aurantiaca</italic> ATCC 27029<sup>T</sup> was selected for whole genome sequencing. Organization of its gene clusters for secondary metabolite biosynthesis revealed 21 clusters encoded to antibiotic production, which is higher than other <italic>Micromonospora</italic> species. Of the genome-predicted antibiotics, kanamycin was found through guided MS/MS analysis producible by the <italic>M. aurantiaca</italic> strain for the first time. The present study highlighted that genomic analysis combined with MS/MS screening is a promising method to discover potential of antibiotic production from rare actinobacteria.</p>