AUTHOR=Mülner Pascal , Schwarz Elisa , Dietel Kristin , Junge Helmut , Herfort Stefanie , Weydmann Max , Lasch Peter , Cernava Tomislav , Berg Gabriele , Vater Joachim 

TITLE=Profiling for Bioactive Peptides and Volatiles of Plant Growth Promoting Strains of the Bacillus subtilis Complex of Industrial Relevance

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01432

DOI=10.3389/fmicb.2020.01432

ISSN=1664-302X

ABSTRACT=<p>Plant growth promoting rhizobacteria attain increasing importance in agriculture as biofertilizers and biocontrol agents. These properties significantly depend on the formation of bioactive compounds produced by such organisms. In our work we investigated the biosynthetic potential of 13 industrially important strains of the <italic>Bacillus subtilis</italic> complex by mass spectrometric methodology. Typing of these organisms was performed with MALDI-TOF mass spectrometry followed by comprehensive profiling of their bioactive peptide products. Volatiles were determined by gas chromatography-mass spectrometry. Representative products of the members of the <italic>B. subtilis</italic> complex investigated in detail were: the surfactin familiy (surfactins, lichenysins, pumilacidins); the iturin family (iturins, mycosubtilins and bacillomycins); plantazolicin and the dual lantibiotics lichenicidins, as well as a wide spectrum of volatiles, such as hydrocarbons (alkanes/alkenes), alcohols, ketones, sulfur-containing compounds and pyrazines. The subcomplexes of the <italic>B. subtilis</italic> organizational unit; (a) <italic>B. subtilis/Bacillus atrophaeus</italic>; (b) <italic>B. amyloliquefaciens/B. velezensis</italic>; (c) <italic>B. licheniformis</italic>, and (d) <italic>B. pumilus</italic> are equipped with specific sets of these compounds which are the basis for the evaluation of their biotechnological and agricultural usage. The 13 test strains were evaluated in field trials for growth promotion of potato and maize plants. All of the implemented strains showed efficient growth stimulation of these plants. The highest effects were obtained with <italic>B. velezensis, B. subtilis</italic>, and <italic>B. atrophaeus</italic> strains.</p>