AUTHOR=Sarveswari Hema Bhagavathi , Kalimuthu Shanthini , Shanmugam Karthi , Neelakantan Prasanna , Solomon Adline Princy 

TITLE=Exploration of Anti-infectives From Mangrove-Derived Micromonospora sp. RMA46 to Combat Vibrio cholerae Pathogenesis

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01393

DOI=10.3389/fmicb.2020.01393

ISSN=1664-302X

ABSTRACT=<p><italic>Vibrio cholerae</italic>, the etiological agent of cholera, employs quorum sensing (QS) pathways to control the expression of virulence factors, including the production of cholera toxin and biofilm formation. Acquired antibiotic resistance in <italic>V. cholerae</italic> draws attention to the development of novel therapeutics that counteract virulence, rather than the viability of the pathogen. In this context, we explored the anti-infective potential of rare marine Actinobacteria (RMA) from a mangrove ecosystem. Here, we report the effects of <italic>Micromonospora</italic> sp. RMA46 against <italic>V. cholerae in vitro</italic>. The RMA46 organic extract was non-bactericidal to <italic>V. cholerae</italic> cells and non-cytotoxic to macrophage RAW264.7 cell lines. RMA46 inhibited the formation of <italic>V. cholerae</italic> biofilms and downregulated the QS global switches LuxO and HapR, as well as other virulence genes including <italic>ct</italic>, <italic>tcp</italic>, and <italic>hap</italic>A. <italic>In silico</italic> molecular docking simulation of RMA46 ethyl acetate extract with LuxO and HapR revealed that 2-methoxy-4-vinylphenol and hexahydro-3-(phenylmethyl)-pyrrolo[1,2-<italic>a</italic>]pyrazine-1,4-dione could interact with the active sites of LuxO and HapR and potentially inhibit them. This study highlights <italic>Micromonospora</italic> sp. RMA46 as a potential source of anti-infectives against <italic>V. cholerae</italic>.</p>