AUTHOR=Li Qiang , He Xiaohui , Ren Yuanhang , Xiong Chuan , Jin Xin , Peng Lianxin , Huang Wenli 

TITLE=Comparative Mitogenome Analysis Reveals Mitochondrial Genome Differentiation in Ectomycorrhizal and Asymbiotic Amanita Species

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01382

DOI=10.3389/fmicb.2020.01382

ISSN=1664-302X

ABSTRACT=<p>In this present study, we assembled and analyzed the mitogenomes of two asymbiotic and six ectomycorrhizal <italic>Amanita</italic> species based on next-generation sequencing data. The size of the eight <italic>Amanita</italic> mitogenomes ranged from 37,341 to 137,428 bp, and we considered introns to be one of the main factors contributing to the size variation of <italic>Amanita</italic>. The introns of the <italic>cox1</italic> gene experienced frequent gain/loss events in <italic>Amanita</italic>; and the intron position class cox1P386 was lost in the six ectomycorrhizal <italic>Amanita</italic> species. In addition, ectomycorrhizal <italic>Amanita</italic> species had more repetitive sequences and fewer intergenic sequences than asymbiotic <italic>Amanita</italic> species in their mitogenomes. Large-scale gene rearrangements were detected in the <italic>Amanita</italic> species we tested, including gene displacements and inversions. On the basis of the combined mitochondrial gene set, we reconstructed the phylogenetic relationships of 66 <italic>Basidiomycetes</italic>. The six ectomycorrhizal <italic>Amanita</italic> species were of single origin, and the two saprophytic <italic>Amanita</italic> species formed two distinct clades. This study is the first to elucidate the functions of the mitogenome in the evolution and ecological adaptation of <italic>Amanita</italic> species.</p>