AUTHOR=Pem Dhandevi , Jeewon Rajesh , Selcuk Faruk , Ulukapi Merve , Bhat Jayarama , Doilom Mingkwan , Lumyong Saisamorn , Hyde Kevin D. 

TITLE=Ribosomal and Protein Gene Phylogeny Reveals Novel Saprobic Fungal Species From Juglans regia and Urtica dioica

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.01303

DOI=10.3389/fmicb.2020.01303

ISSN=1664-302X

ABSTRACT=<p>During an ongoing investigation of Ascomycetes from plant substrates, three saprobic species were found from plant substrates. Two new species, <italic>Leptosphaeria regiae</italic> and <italic>Neomicrosphaeropsis juglandis</italic> were isolated from dead branches of <italic>Juglans regia</italic> from Turkey. Another species is introduced herein as <italic>Subplenodomus urticae</italic> sp. nov within the family Leptosphaeriaceae found on <italic>Urtica dioica</italic> in Italy. Multigene phylogenies based on combined LSU, ITS, SSU, and β-tubulin DNA sequence data generated from maximum likelihood and MrBayes analyses indicate that <italic>Leptosphaeria regiae</italic> is related to <italic>L. slovacica</italic> and forms an independent lineage within the genus <italic>Leptosphaeria</italic>. <italic>Subplenodomus urticae</italic> is basal to <italic>S. iridicola</italic> and its establishment as a new species is strongly supported. <italic>Neomicrosphaeropsis juglandis</italic> forms a moderately supported lineage in between <italic>N. italica</italic> and <italic>N. elaeagni</italic> in the Didymellaceae. Full morphological details are provided herein and phylogenetic relationships of the three new species are also discussed.</p>