AUTHOR=Mokhtar Nur Fadhilah Khairil , Hashim Amalia Mohd , Hanish Irwan , Zulkarnain Aisyah , Raja Nhari Raja Mohd Hafidz , Abdul Sani Asmahani Azira , Abbasiliasi Sahar , Ariff Arbakariya , Mustafa Shuhaimi , Rahim Raha Abdul 

TITLE=The Discovery of New Antilisterial Proteins From Paenibacillus polymyxa Kp10 via Genome Mining and Mass Spectrometry

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00960

DOI=10.3389/fmicb.2020.00960

ISSN=1664-302X

ABSTRACT=<p>The inhibitory properties of novel antimicrobial proteins against food-borne pathogens such as <italic>Listeria monocytogenes</italic> offer extensive benefits to the food and medical industries. In this study, we have identified antimicrobial proteins from a milk curd–derived bacterial isolate that exhibits antilisterial activity using genome mining and mass spectrometry analysis. The analysis of the draft genome sequence identified the isolate as <italic>Paenibacillus polymyxa</italic> Kp10, and predicted the presence of antimicrobial paenibacillin, paenilan, paeninodin, sactipeptides, thiazole-oxazole modified microcin, and histone-like DNA binding protein HU encoded in its genome. Interestingly, nanoLC-MS/MS analysis identified two histone-like DNA binding proteins HU as predicted <italic>in silico</italic> earlier, exhibiting antilisterial activity. Additionally, translation initiation factor IF-1 and 50S ribosomal protein L29 were also discovered by the mass spectrometry in the active fractions. The antilisterial activity of the four proteins was verified through heterologous protein expression and antimicrobial activity assay <italic>in vitro</italic>. This study has identified structural regulatory proteins from <italic>Paenibacillus</italic> possessing antilisterial activity with potential future application in the food and medical industries.</p>