AUTHOR=Li Yingbin , Liu Yixiang , Zhang Zhiping , Cao Yongsong , Li Jianqiang , Luo Laixin 

TITLE=Allyl Isothiocyanate (AITC) Triggered Toxicity and FsYvc1 (a STRPC Family Member) Responded Sense in Fusarium solani

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00870

DOI=10.3389/fmicb.2020.00870

ISSN=1664-302X

ABSTRACT=<p>Allyl isothiocyanate (AITC) is a natural product used as a food additive. Due to its strong volatility and broad biological activity, AITC is considered as a bio-fumigant to control soil-borne fungal diseases in agriculture, creating an urgent need for evaluation of the antifungal activity of AITC. Here we study the effect of AITC on <italic>Fusarium solani</italic> growth and explore the molecular mechanisms. The results indicated that AITC causes rapid inhibition of <italic>F</italic>. <italic>solani</italic> after 5 min, hyphal deformity, and electrolyte leakage. A yeast-like vacuolar transient receptor potential channel regulator (<italic>FsYvc1</italic>, a STRPC family member) was identified in <italic>F</italic>. <italic>solani</italic> that seems to play a role in this fungi AITC sensitivity. Genetic evidence suggests the gene <italic>FsYvc1</italic> is involved in <italic>F</italic>. <italic>solani</italic> growth, development, and pathogenicity. Loss of <italic>FsYvc1</italic> resulted in hypersensitivity of <italic>F</italic>. <italic>solani</italic> to AITC and induced reactive oxygen species (ROS) accumulation ∼ 1.3 to 1.45- folds that of the wild type (WT), and no difference responses to CaCl<sub>2</sub>, NaCl, KCl, SDS, and Congo red when compared with WT. In addition, Δ<italic>FsYvc1-17</italic> showed significantly reduced (∼ 1-fold) glutathione-S-transferase (GST) expression compared with the WT without AITC induction. Upon exposure to 4.8 μg/mL AITC for 3 h, the relative expression levels were ∼ 12–30 fold higher in both the WT and Δ<italic>FsYvc1-17</italic>. Nevertheless, no difference in GST expression level was observed between the WT and Δ<italic>FsYvc1-17</italic>. The current study provides novel insights into the toxicity mechanisms of AITC. Considering our results that show the key role of <italic>FsYvc1</italic>, we propose that it could act as a new molecular target for future fungicide development.</p>