AUTHOR=Song Nan , Geng Yuehua , Li Xinghao 

TITLE=The Mitochondrial Genome of the Phytopathogenic Fungus Bipolaris sorokiniana and the Utility of Mitochondrial Genome to Infer Phylogeny of Dothideomycetes

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00863

DOI=10.3389/fmicb.2020.00863

ISSN=1664-302X

ABSTRACT=<p>A number of species in <italic>Bipolaris</italic> are important plant pathogens. Due to a limited number of synapomorphic characters, it is difficult to perform species identification and to estimate phylogeny of <italic>Bipolaris</italic> based solely on morphology. In this study, we sequenced the complete mitochondrial genome of <italic>Bipolaris sorokiniana</italic>, and presented the detailed annotation of the genome. The <italic>B. sorokiniana</italic> mitochondrial genome is 137,775 bp long, and contains two ribosomal RNA genes, 12 core protein-coding genes, 38 tRNA genes. In addition, two ribosomal protein genes (<italic>rps3</italic> gene and <italic>rps5</italic> gene) and the fungal mitochondrial RNase P gene (<italic>rnpB</italic>) are identified. The large genome size is mostly determined by the presence of numerous intronic and intergenic regions. A total of 28 introns are inserted in eight core protein-coding genes. Together with the published mitochondrial genome sequences, we conducted a preliminary phylogenetic inference of Dothideomycetes under various datasets and substitution models. The monophyly of <italic>Capnodiales</italic>, <italic>Botryosphaeriales</italic> and <italic>Pleosporales</italic> are consistently supported in all analyses. The <italic>Venturiaceae</italic> forms an independent lineage, with a distant phylogenetic relationship to <italic>Pleosporales</italic>. At the family level, the <italic>Mycosphaerellaceae</italic>, <italic>Botryosphaeriaceae</italic>. <italic>Phaeosphaeriaceae</italic>, and <italic>Pleosporaceae</italic> are recognized in the majority of trees.</p>