AUTHOR=Wang Gangzheng , Luo Yi , Wang Chen , Zhou Yan , Mou Chunye , Kang Heng , Xiao Yang , Bian Yinbing , Gong Yu Hua 

TITLE=Hsp40 Protein LeDnaJ07 Enhances the Thermotolerance of Lentinula edodes and Regulates IAA Biosynthesis by Interacting LetrpE

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00707

DOI=10.3389/fmicb.2020.00707

ISSN=1664-302X

ABSTRACT=<p>Our previous study found that <italic>LeDnaJ07</italic> RNAi decreased <italic>Lentinula edodes</italic> resistance to heat stress and <italic>Trichoderma atroviride</italic> infection. In this study, the structure and function of the <italic>LeDnaJ07</italic> gene was analyzed by gene cloning and overexpression in <italic>L. edodes</italic> stress-sensitive strain YS55 via the <italic>Agrobacterium</italic>-mediated transformation method. Transformants were confirmed by qRT-PCR, fluorescence observation and Southern blotting. Overexpression of <italic>LeDnaJ07</italic> in YS55 not only enhanced <italic>L. edodes</italic> mycelial resistance to heat stress but also facilitated mycelial growth. In the presence of heat stress, the intracellular IAA content showed a significant increase in the two <italic>LeDnaJ07</italic> overexpression strains but only a slight change in the YS55 wild type strain. Moreover, the interaction between LeDnaJ07 and LetrpE was demonstrated via Y2H and BiFC assays. These results suggested that LeDnaJ07 may be involved in regulating IAA biosynthesis and the resistance of <italic>L. edodes</italic> to heat stresses via interacting with LetrpE.</p>