AUTHOR=Wang Xuewei , Zhao Zhibai , Tang Nan , Zhao Yuping , Xu Juanyong , Li Liuyang , Qian Ling , Zhang Junfeng , Fan Yuan 

TITLE=Microbial Community Analysis of Saliva and Biopsies in Patients With Oral Lichen Planus

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00629

DOI=10.3389/fmicb.2020.00629

ISSN=1664-302X

ABSTRACT=<p>The specific etiology and pathogenesis of oral lichen planus (OLP) remain elusive, and microbial dysbiosis may play an important role in OLP. We evaluated the saliva and tissue bacterial community of patients with OLP and identified the colonization of bacteria in OLP tissues. The saliva (<italic>n</italic> = 60) and tissue (<italic>n</italic> = 24) samples from OLP patients and the healthy controls were characterized by 16S rDNA gene sequencing and the bacterial signals in OLP tissues were detected by fluorescence <italic>in situ</italic> hybridization (FISH) targeting the bacterial 16S rDNA gene. Results indicate that the OLP tissue microbiome was different from the microbiota of OLP saliva. Compared with the healthy controls, <italic>Capnocytophaga</italic> and <italic>Gemella</italic> were higher in OLP saliva, while <italic>Escherichia</italic>–<italic>Shigella</italic> and <italic>Megasphaera</italic> were higher in OLP tissues, whereas seven taxa, including Carnobacteriaceae, Flavobacteriaceae, and <italic>Megasphaera</italic>, were enriched in both saliva and tissues of OLP patients. Furthermore, FISH found that the average optical density (AOD) of bacteria in the lamina propria of OLP tissues was higher than that of the healthy controls, and the AOD of bacteria in OLP epithelium and lamina propria was positively correlated. These data provide a different perspective for future investigation on the OLP microbiome.</p>