AUTHOR=Chen Zhiyu , Ou Peiyu , Liu Lingyan , Jin Xiaobao 

TITLE=Anti-MRSA Activity of Actinomycin X2 and Collismycin A Produced by Streptomyces globisporus WA5-2-37 From the Intestinal Tract of American Cockroach (Periplaneta americana)

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00555

DOI=10.3389/fmicb.2020.00555

ISSN=1664-302X

ABSTRACT=<p>Methicillin-resistant <italic>Staphylococcus aureus</italic> (MRSA) is recognized as one of the serious pathogen that causes acquired infections worldwide. Its emerging need to discover novel, safe and potent anti-MRSA drugs. In this study, primary screening by anti-MRSA activity assay found one strain WA5-2-37 isolated from the intestinal tract of <italic>Periplaneta americana</italic>, exhibited great activity against MRSA ATCC 43300. The strain WA5-2-37 produced actinomycin X<sub>2</sub> and collismycin A which showed strong inhibition of MRSA with minimum inhibitory concentration (MIC) values of 0.25 and 8 μg/mL. The structures of the pure compounds were elucidated by analysis of mass spectrometry (MS), <sup>1</sup>H and <sup>13</sup>C nuclear magnetic resonance (NMR). The strain WA5-2-37 was considered as <italic>Streptomyces globisporus</italic> on the basis of morphological characteristics, genotypic data, and phylogenetic analysis. This is the first reported naturally occurring strain of <italic>S. globisporus</italic> isolated from the intestinal tract of <italic>P. americana</italic>, whereas it has almost been found from plants, marine, and soil previously. Moreover, <italic>S. globisporus</italic> has not been reported to produce any anti-MRSA substances previously, such as actinomycin X<sub>2</sub> and collismycin A. In conclusion, the insect-derived strain of <italic>S. globisporus</italic> WA5-2-37 was considered of great potential as a new strain of producing actinomycin X<sub>2</sub>, collismycin A or other anti-MRSA compounds.</p>