AUTHOR=Nagy Tibor , Szmolka Ama , Wilk Tímea , Kiss János , Szabó Mónika , Pászti Judit , Nagy Béla , Olasz Ferenc 

TITLE=Comparative Genome Analysis of Hungarian and Global Strains of Salmonella Infantis

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00539

DOI=10.3389/fmicb.2020.00539

ISSN=1664-302X

ABSTRACT=<sec><title>Introduction</title><p>The emergence and spread of new strains of zoonotic bacteria, such as multidrug resistant (MDR) <italic>Salmonella</italic> Infantis, represent a growing health risk for humans in and outside Europe due to foodborne infections of poultry meat origin.</p></sec><sec><title>Objectives</title><p>In order to understand genome relations of <italic>S</italic>. Infantis strains from Hungary and from different geographic regions, we performed a comprehensive genome analysis of nine Hungarian and 67 globally selected strains of <italic>S</italic>. Infantis and 26 <italic>Salmonella</italic> strains representing 13 non-Infantis serovars.</p></sec><sec><title>Results</title><p>Analyses of whole-, and accessory genomes, showed that almost all <italic>S</italic>. Infantis strains were separated from the non-Infantis serovars. <italic>S</italic>. Infantis strains from Hungary formed subclusters based on their time of isolation. In whole genome sequence analysis, the Swiss strains of <italic>S</italic>. Infantis were closely related to each other and clustered together with subclusters of strains from Hungary, Japan, Italy, United States, and Israel. The accessory genome analysis revealed that the Swiss strains were distinct from most of the strains investigated, including the Hungarian ones. Analysis of the cloud genes offered the most detailed insight into the genetic distance and relationship of <italic>S</italic>. Infantis strains confirming that the Swiss and Hungarian strains belonged to different lineages. As expected, core genome analysis provided the least discriminatory power for analysis of <italic>S</italic>. Infantis. Genomic sequences of nine strains from Brazil, Israel, Mexico, Nigeria, and Senegal (deposited as <italic>S</italic>. Infantis) proved to be outliers from the <italic>S</italic>. Infantis clade. They were predicted to be <italic>Salmonella</italic> Rissen, <italic>Salmonella</italic> Ouakarm, <italic>Salmonella</italic> Kentucky, <italic>Salmonella</italic> Thompson, and <italic>Salmonella</italic> enterica subsp. <italic>diarizonae</italic>.</p></sec><sec><title>Conclusion</title><p>Accessory genome of <italic>S</italic>. Infantis showed the highest diversity suggesting a faster evolution than that of the whole genomes contributing to the emergence of multiple genetic variants of <italic>S</italic>. Infantis worldwide. Accordingly, in spite of the comprehensive analysis of several genomic characteristics, no epidemiologic links between these <italic>S</italic>. Infantis strains from different countries could be established. It is also concluded that several strains originally designated as <italic>S</italic>. Infantis need in databanks reclassification.</p></sec>