AUTHOR=Kosaka Tomoyuki , Nishioka Aya , Sakurada Tomoko , Miura Kento , Anggarini Sakunda , Yamada Mamoru TITLE=Enhancement of Thermal Resistance by Metal Ions in Thermotolerant Zymomonas mobilis TISTR 548 JOURNAL=Frontiers in Microbiology VOLUME=11 YEAR=2020 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00502 DOI=10.3389/fmicb.2020.00502 ISSN=1664-302X ABSTRACT=

The thermal resistance of fermenting microbes is a key characteristic of stable fermentation at high temperatures. Therefore, the effects of various metal ions on the growth of Zymomonas mobilis TISTR 548, a thermotolerant ethanologenic bacterium, at a critical high temperature (CHT) were examined. Addition of Mg²⁺ and K⁺ increased CHT by 1°C, but the effects of the addition of Mn²⁺, Ni²⁺, Co²⁺, Al³⁺, Fe³⁺, and Zn²⁺ on CHT were negligible. To understand the physiological functions associated with the addition of Mg²⁺ or K⁺, cell morphology, intracellular reactive oxygen species (ROS) level, and ethanol productivity were investigated at 39°C (i.e., above CHT). Cell elongation was repressed by Mg²⁺, but not by K⁺. Addition of both metals reduced intracellular ROS level, with only K⁺ showing the highest reduction strength, followed by both metals and only Mg²⁺. Additionally, ethanol productivity was recovered with the addition of both metals. Moreover, the addition of Mg²⁺ or K⁺ at a non-permissive temperature in 26 thermosensitive, single gene-disrupted mutants of Z. mobilis TISTR 548 revealed that several mutants showed metal ion-specific growth improvement. Remarkably, K⁺ repressed growth of two mutants. These results suggest that K⁺ and Mg²⁺ enhance cell growth at CHT via different mechanisms, which involve the maintenance of low intracellular ROS levels.