AUTHOR=Zhang Yawei , Li Fuyong , Chen Yanhong , Wu Hao , Meng Qingxiang , Guan Le Luo 

TITLE=Metatranscriptomic Profiling Reveals the Effect of Breed on Active Rumen Eukaryotic Composition in Beef Cattle With Varied Feed Efficiency

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00367

DOI=10.3389/fmicb.2020.00367

ISSN=1664-302X

ABSTRACT=<p>Exploring the compositional characteristics of rumen eukaryotic community can expand our understanding of their role in rumen function and feed efficiency. In this study, we applied metatranscriptomics to characterize the active rumen eukaryotic community (protozoa and fungi) in beef cattle (<italic>n</italic> = 48) of three breeds [Angus (AN), Charolais (CH), and Kinsella Composite (KC)] and with divergent residual feed intake (RFI). The composition of active rumen eukaryotic microbiota was evaluated based on enriched 18S rRNAs from the metatranscriptomic datasets. At the phylum level, a total of four protozoal taxa (<italic>Ciliophora</italic>, <italic>Parabasalia</italic>, unclassified <italic>SAR</italic>, and unclassified <italic>Alveolata</italic>), six fungal taxa (<italic>Neocallimastigomycota</italic>, <italic>Basidiomycota</italic>, unclassified <italic>Fungi</italic>, <italic>Mucoromycota</italic>, <italic>Ascomycota</italic>, and <italic>Chytridiomycota</italic>), and one sister group of kingdom <italic>Fungi</italic> (unclassified <italic>Opisthokonta</italic>) were detected with relative abundances higher than 0.01% and in at least 50% of animals within each breed. Among these, <italic>Ciliophora</italic>, <italic>Parabasalia</italic>, unclassified <italic>Opisthokonta</italic>, and <italic>Neocallimastigomycota</italic> were the top four active eukaryotic phyla. At the genus level, a total of 8 ciliated protozoa, 5 flagellated protozoa, 5 anaerobic fungi, and 10 aerobic fungi taxa were detected, with unclassified <italic>Trichostomatia</italic>, <italic>Tetratrichomonas</italic>, unclassified <italic>Neocallimastigaceae</italic>, and <italic>Pleurotus</italic> being the most predominant taxa of ciliated protozoa, flagellated protozoa, anaerobic fungi, and aerobic fungi, respectively. Differential abundance analysis revealed that breed had a significant effect on the phylogenetic lineages of rumen eukaryotes, and seven fungal taxa were more abundant (linear discriminant analysis score &gt; 2 with <italic>P</italic> &lt; 0.05) in the rumen of KC steers than in the rumen of AN and CH steers. Although principal coordinate analysis (PCoA) revealed that the ruminal active eukaryotic profiles were not distinguishable between high- and low-RFI groups, the diversity indices, including Faith’s phylogenetic diversity (PD), observed operational taxonomic units (OTUs), and Shannon index of rumen eukaryotes were higher in low-RFI steers than those in high-RFI steers. Meanwhile, the abundance of genus <italic>Entodinium</italic> and the kingdom <italic>Fungi</italic> was higher in low-RFI steers than that in high-RFI steers. This information on active rumen eukaryotic microbiota and identified differential abundance of taxa between high- and low-RFI animals suggests the possibility of improving feed efficiency through altering rumen eukaryotic microbiota.</p>