AUTHOR=Li Shangyong , Wang Linna , Jung Samil , Lee Beom Suk , He Ningning , Lee Myeong-Sok 

TITLE=Biochemical Characterization of a New Oligoalginate Lyase and Its Biotechnological Application in Laminaria japonica Degradation

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00316

DOI=10.3389/fmicb.2020.00316

ISSN=1664-302X

ABSTRACT=<p>Oligoalginate lyases catalyze the degradation of alginate polymers and oligomers into monomers, a prerequisite for biotechnological utilizing alginate. In this study, we report the cloning, expression and biochemical characterization of a new polysaccharide lyase (PL) family 17 oligoalginate lyase, OalV17, from the marine bacterium <italic>Vibrio</italic> sp. SY01. The recombinant OalV17 showed metal ion independent and detergent resistant properties. Furthermore, OalV17 is an exo-type enzyme that yields alginate monomers as the main product and recognizes alginate disaccharides as the minimal substrate. Site-directed mutagenesis followed by kinetic analysis indicates that the residue Arg<sup>231</sup> plays a key role in substrate specificity. Furthermore, a rapid and efficient alginate monomer-producing method was developed directly from <italic>Laminaria japonica</italic>. These results suggest that OalV17 is a potential candidate for saccharification of alginate.</p>