AUTHOR=Zhang Jing , Gao Cheng , Yu Xue-Mei , Lun He-Yuan , Du Zong-Jun 

TITLE=Chryseobacterium lacus sp. nov. Isolated From the Surface Water of Two Lakes With Light-Induced Carotenoid Production

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00251

DOI=10.3389/fmicb.2020.00251

ISSN=1664-302X

ABSTRACT=<p>Two Gram-stain-negative, rod-shaped, gliding, catalase-positive, and facultative anaerobic strains, YLOS41<sup>T</sup> and XH07, were isolated from surface water of Yilong Lake and West Lake of Dali in Yunnan Province, respectively. Both strains were yellow-colored under light conditions and white-colored under dark conditions. The results of physiological and chemotaxonomic characterization, sequencing and phylogenetic analysis, and draft genome sequence comparison demonstrated that the two strains represented a single novel species within the genus <italic>Chryseobacterium</italic>, for which the name <italic>Chryseobacterium lacus</italic> sp. nov. is proposed. The type strain is YLOS41<sup>T</sup> (= KCTC 62352<sup>T</sup> = MCCC 1H00300<sup>T</sup>), and the second strain is XH07 (= KCTC 62993). During the cultivation process, we found that the colony color of the two strains changed from white to yellow with illumination. The study investigated the effects of light irradiation on the strain YLOS41<sup>T</sup>. Results showed that light irradiation did not affect the growth of cells but significantly increased carotenoid synthesis, which caused the change of colony color. In-depth metabolic analysis was conducted by transcriptome. The predominant changes were found for genes involved in carotenoid synthesis as protection from light damage. Based on the genome and transcriptome, we proved that strain YLOS41<sup>T</sup> possessed a complete synthetic pathway of carotenoid and speculated that the production was zeaxanthin. This was the first report of <italic>Chryseobacterium</italic> species with light-induced carotenoid synthesis. This study enhances our present knowledge on how <italic>Chryseobacterium</italic> species isolated from surface water responds to light damage.</p>