AUTHOR=Zhong Xian , Wu Qingping , Zhang Jumei , Ma Zonghao , Wang Juan , Nie Xiang , Ding Yu , Xue Liang , Chen Moutong , Wu Shi , Wei Xianhu , Zhang Youxiong 

TITLE=Campylobacter jejuni Biofilm Formation Under Aerobic Conditions and Inhibition by ZnO Nanoparticles

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00207

DOI=10.3389/fmicb.2020.00207

ISSN=1664-302X

ABSTRACT=<p><italic>Campylobacter jejuni</italic> is a major foodborne pathogen worldwide. As it forms biofilms, it can become a persistent contaminant in the food and pharmaceutical industries. In this study, it was demonstrated that <italic>C. jejuni</italic> could make more biofilm in aerobic conditions than in microaerobic conditions, and only 13.9% <italic>C. jejuni</italic> entered coccus (a VBNC state) under microaerobic conditions; however, the rate increased to 95.5% under aerobic conditions. <italic>C. jejuni</italic> could form more biofilm in mixed culture with <italic>Escherichia coli</italic> or <italic>Pseudomonas aeruginosa</italic> than in pure culture. Scanning electron microscope results showed that <italic>C. jejuni</italic> retained its normal spiral shape under aerobic conditions for 48 h by forming crosslinks with the aerobic and facultative anaerobic bacteria. Additionally, culture medium containing 0.5 mg/ml ZnO nanoparticles inhibited biofilm formation. Our results provide information on a new approach to controlling contamination via <italic>C. jejuni.</italic></p>