AUTHOR=Petri Renee Maxine , Neubauer Viktoria , Humer Elke , Kröger Iris , Reisinger Nicole , Zebeli Qendrim 

TITLE=Feed Additives Differentially Impact the Epimural Microbiota and Host Epithelial Gene Expression of the Bovine Rumen Fed Diets Rich in Concentrates

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00119

DOI=10.3389/fmicb.2020.00119

ISSN=1664-302X

ABSTRACT=<p>The success of nutritional strategies for the prevention of subacute ruminal acidosis (SARA) and the related microbial dysbiosis still remains unpredictable due to the complexity of the rumen ecosystem. The rumen epimural community, due to proximity, has the greatest opportunity to influence host gene expression. The aim of this study was to determine the effect of two separate feed additives on the rumen epimural community and host epithelial gene expression. Eight rumen cannulated Holstein cows were randomly assigned to one of three feeding groups: autolyzed yeast (AY), phytogenics (PHY) and control (CON) using a 3 × 3 Latin square design. Cows were fed an intermittent SARA model that started with 100% forage diet (Baseline) followed by two 65% concentrate-diet induced SARA challenges (SARAI, SARAII), separated by 1 week of forage only feeding. Rumen papillae samples were collected via the cannula during the Baseline, SARAI and SARAII periods. Microbial DNA was extracted and sequenced targeting the 16S rRNA gene and host RNA was analyzed using RT-qPCR. Analysis of the taxonomic composition at the genera level showed a tendency to increase in the relative abundances of <italic>Pseudobutyrivibrio</italic> (<italic>P</italic> = 0.06), <italic>Selenomonas</italic> (<italic>P</italic> = 0.07) and significantly increase in SHD-231 (<italic>P</italic> = 0.01) in PHY treated animals, whereas <italic>Succiniclasticum</italic> tended to decrease in both PHY and AY treated animals compared to the control. Linear discriminant analysis effect size testing was performed and based on treatment × feeding phase interaction, a number of biomarker genera were identified including the previously identified <italic>Succiniclasticum</italic>. Supplementation with AY correlated positively with <italic>CD14</italic> and <italic>DRA</italic> expression and negatively to <italic>CLDN1</italic>, <italic>MyD88</italic>, and <italic>MCT4</italic> expression. Supplementation with PHY showed a negative correlation to <italic>CLDN4</italic> gene expression. <italic>Anaerovibrio</italic> showed the highest positive Pearson correlations to biogenic amines tested in the rumen fluid including putrescine (<italic>r</italic> = 0.67), cadaverine (<italic>r</italic> = 0.84), and tyramine (<italic>r</italic> = 0.83). These results show that supplementing feed additives to high grain diets can have a positive influence on the stability of the epimural populations, and that changes in the epimural community are correlated with changes in host epithelial gene expression.</p>