AUTHOR=Guo Wei , Gao Jie , Wang Hong-Jie , Su Ru-Yi , Sun Chu-Yun , Gao Si-Han , Liu Jian-Zhong , Chen Gong-You 

TITLE=Phosphoglycerate Kinase Is Involved in Carbohydrate Utilization, Extracellular Polysaccharide Biosynthesis, and Cell Motility of Xanthomonas axonopodis pv. glycines Independent of Clp

JOURNAL=Frontiers in Microbiology

VOLUME=11

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2020.00091

DOI=10.3389/fmicb.2020.00091

ISSN=1664-302X

ABSTRACT=<p>Phosphoglycerate kinase (Pgk), catalyzing the reversible conversions between glycerate-1.3-2P and glycerate-3P, plays an important role in carbohydrate metabolism. Here, we show that a Pgk-deficient mutant (NΔ<italic>pgk</italic>) of <italic>Xanthomonas axonopodis</italic> pv. <italic>glycines</italic> (<italic>Xag</italic>) could grow in medium with glucose, galactose, fructose, mannose, or sucrose, as the sole carbon source, suggesting that <italic>Xag</italic> may employ Entner-Doudoroff (ED) and pentose phosphate pathway (PPP), but not glycolysis, to catabolize glucose. NΔ<italic>pgk</italic> could not utilize pyruvate, suggesting that Pgk might be essential for gluconeogenesis. Mutation in <italic>pgk</italic> led to a reduction of extracellular polysaccharide (EPS) biosynthesis, cell motility, and intracellular ATP. As a result, the virulence of NΔ<italic>pgk</italic> was significantly compromised in soybean. NΔ<italic>pgk</italic> could be fully complemented by the wild-type <italic>pgk</italic>, but not by <italic>clp</italic> (encoding Crp-like protein). qRT-PCR analyses demonstrated that <italic>pgk</italic> is regulated by the HrpG/HrpX cascade, but not by Clp. These results suggest that Pgk is involved in carbohydrate utilization, EPS biosynthesis, and cell motility of <italic>Xag</italic> independent of Clp.</p>