AUTHOR=Engel Franziska , Ossipova Elena , Jakobsson Per-Johan , Vockenhuber Michael-Paul , Suess Beatrix 

TITLE=sRNA scr5239 Involved in Feedback Loop Regulation of Streptomyces coelicolor Central Metabolism

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.03121

DOI=10.3389/fmicb.2019.03121

ISSN=1664-302X

ABSTRACT=<p>In contrast to transcriptional regulation, post-transcriptional regulation and the role of small non-coding RNAs (sRNAs) in streptomycetes are not well studied. Here, we focus on the highly conserved sRNA scr5239 in <italic>Streptomyces coelicolor</italic>. A proteomics approach revealed that the sRNA regulates several metabolic enzymes, among them phosphoenolpyruvate carboxykinase (PEPCK), a key enzyme of the central carbon metabolism. The sRNA scr5239 represses <italic>pepck</italic> at the post-transcriptional level and thus modulates the intracellular level of phosphoenolpyruvate (PEP). The expression of scr5239 in turn is dependent on the global transcriptional regulator DasR, thus creating a feedback loop regulation of the central carbon metabolism. By post-transcriptional regulation of PEPCK and in all likelihood other targets, scr5239 adds an additional layer to the DasR regulatory network and provides a tool to control the metabolism dependent on the available carbon source.</p>