AUTHOR=Xie Meihua , Bai Na , Yang Jiangliu , Jiang Kexin , Zhou Duanxu , Zhao Yining , Li Dongni , Niu Xuemei , Zhang Ke-Qin , Yang Jinkui 

TITLE=Protein Kinase Ime2 Is Required for Mycelial Growth, Conidiation, Osmoregulation, and Pathogenicity in Nematode-Trapping Fungus Arthrobotrys oligospora

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.03065

DOI=10.3389/fmicb.2019.03065

ISSN=1664-302X

ABSTRACT=<p>Inducer of meiosis 2 (Ime2), a protein kinase that has been identified in diverse fungal species, functions in the regulation of various cellular processes, such as ascospore formation, pseudohyphal growth, and sexual reproduction. In this study, AoIme2, an ortholog of <italic>Saccharomyces cerevisiae</italic> Ime2, was characterized in the nematode-trapping fungus <italic>Arthrobotrys oligospora</italic>. Disruption of the gene <italic>Aoime2</italic> caused defective growth, with slower mycelial growth in Δ<italic>Aoime2</italic> mutants than the wild type (WT) strain, and in the mutants, the number of hyphal septa in mycelia was higher and the number of cell nuclei in mycelia and conidia was considerably lower than in the WT strain. The conidial yields of the Δ<italic>Aoime2</italic> mutants were decreased by ∼33% relative to the WT strain, and the transcription of several sporulation-related genes, including <italic>abaA</italic>, <italic>fluG</italic>, <italic>rodA</italic>, <italic>aspB</italic>, <italic>velB</italic>, and <italic>vosA</italic>, was markedly downregulated during the conidiation stage. The Δ<italic>Aoime2</italic> mutants were highly sensitive to the osmotic stressors NaCl and sorbitol, and the cell wall of partial hyphae in the mutants was deformed. Further examination revealed that the cell wall of the traps produced by Δ<italic>Aoime2</italic> mutants became loose, and that the electron-dense bodies in trap cells were also few than in the WT strain. Moreover, <italic>Aoime2</italic> disruption caused a reduction in trap formation and serine-protease production, and most hyphal traps produced by Δ<italic>Aoime2</italic> mutants did not form an intact hyphal loop; consequently, substantially fewer nematodes were captured by the mutants than by the WT strain. In summary, an Ime2-MAPK is identified here for the first time from a nematode-trapping fungus, and the kinase is shown to be involved in the regulation of mycelial growth and development, conidiation, osmolarity, and pathogenicity in <italic>A. oligospora</italic>.</p>