AUTHOR=Cordero-Bueso Gustavo , Moraga Javier , Ríos-Carrasco María , Ruiz-Muñoz Marina , Cantoral Jesús Manuel 

TITLE=Bacteriophages as an Up-and-Coming Alternative to the Use of Sulfur Dioxide in Winemaking

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2020

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02931

DOI=10.3389/fmicb.2019.02931

ISSN=1664-302X

ABSTRACT=<p>Certain acetic and lactic acid bacteria are major causes of quality defects in musts and wines, giving rise to defects such as a “vinegary,” “sharp, like nail polish-remover” taste or preventing alcoholic and/or malolactic fermentation. Sulfur dioxide is the major tool currently used in the control of these bacteria in wine. The aim of this work was to isolate bacteriophages from musts and wine of different grape varieties that were able to eliminate lactic and acetic acid bacteria spoilages at the laboratory scale. Musts obtained from grape-berries of <italic>Vitis vinifera</italic> cv. Chardonnay and Moscatel and a red wine made with <italic>V. vinifera</italic> cv. Tintilla de Rota were used to isolate bacteriophages. Bacteriophages were obtained from each of the musts and the wine and belonged to the order <italic>Caudovirals</italic> and the family <italic>Tectivirals</italic>. They were isolated by classical virology methods and identified by electron microscopy. The host bacteria used in the study were lactic acid bacteria of the species <italic>Lactobacillus hilgardii</italic>, <italic>Lactobacillus plantarum</italic>, and <italic>Oenococcus oeni</italic> and the acetic bacteria <italic>Acetobacter aceti</italic>. A comparative study was performed by adding phage titrations and SO<sub>2</sub> to musts and wines, which had been previously inoculated with bacteria, to study the effectiveness of bacteriophages against bacteria. The comparative study showed that some bacteriophages were as effective as sulfur dioxide at low concentrations.</p>