AUTHOR=Zhang Qi-Lin , Li Hong-Wei , Wu Wei , Zhang Man , Guo Jun , Deng Xian-Yu , Wang Feng , Lin Lian-Bing 

TITLE=The Response of Microbiota Community to Streptococcus agalactiae Infection in Zebrafish Intestine

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02848

DOI=10.3389/fmicb.2019.02848

ISSN=1664-302X

ABSTRACT=<p>Recently, <italic>Streptococcus agalactiae</italic> has become a major pathogen leading to Streptococcosis. To understand the physiological responses of zebrafish (<italic>Danio rerio</italic>) to <italic>S. agalactiae</italic>, the intestinal microbiota composition of the intestine (12 and 24 h post-infection, hpi, respectively) in zebrafish infected with <italic>S. agalactiae</italic> were investigated. The intestinal bacterial composition was analyzed using PacBio high-throughput full-length 16S rRNA gene sequencing. The most predominant bacteria in the zebrafish intestine were the Fusobacteria phylum and <italic>Sphingomonas</italic> genus. <italic>S. agalactiae</italic> infection affected the composition of partially intestinal microbiota. At the species level, the relative abundance of the pathogenic intestinal bacteria <italic>Aeromonas veronii</italic>, <italic>S. agalactiae</italic>, and <italic>Clostridium tarantellae</italic> significantly increased after <italic>S. agalactiae</italic> infection (<italic>p</italic> &lt; 0.05), while that of the beneficial intestinal bacteria <italic>Bacillus licheniformis</italic>, <italic>Comamonas koreensis</italic>, and <italic>Romboutsia ilealis</italic> significantly decreased (<italic>p</italic> &lt; 0.05), showing that <italic>S. agalactiae</italic> infection aggravates the zebrafish disease through promoting abundance of other intestinal pathogenic bacteria. This study is the first PacBio analyses of the zebrafish intestinal microbiota community under pathogenic infection. Results suggest that the <italic>S. agalactiae</italic> infection alters the intestinal flora structure in zebrafish.</p>