AUTHOR=Huang Liming , Ding Lijian , Li Xiaohui , Wang Ning , Cui Wei , Wang Xiao , Naman C. Benjamin , Lazaro J. Enrico H. , Yan Xiaojun , He Shan 

TITLE=New Dihydroisocoumarin Root Growth Inhibitors From the Sponge-Derived Fungus Aspergillus sp. NBUF87

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02846

DOI=10.3389/fmicb.2019.02846

ISSN=1664-302X

ABSTRACT=<p>Six new dihydroisocoumarins, aspergimarins A−F (<bold>1</bold>−<bold>6</bold>), were discovered together with five known analogs (<bold>7</bold>−<bold>11</bold>) from a monoculture of the sponge-derived fungus <italic>Aspergillus</italic> sp. NBUF87. The structures of these compounds were elucidated through comprehensive spectroscopic methods, and absolute configurations were assigned after X-ray crystallography, use of the modified Mosher’s method, and comparison of electronic circular dichroism (ECD) data with literature values for previously reported analogs. Compounds <bold>1</bold>−<bold>11</bold> were evaluated in a variety of bioassays, and at 100 μM, both <bold>1</bold> and <bold>5</bold> showed significant inhibitory effects on the lateral root growth of <italic>Arabidopsis thaliana</italic> Columbia-0 (Col-0). Moreover, at 100 μM, <bold>5</bold> also possessed notable inhibition against the primary root growth of Col-0. Meanwhile, <bold>1</bold>−<bold>11</bold> were all found to be inactive <italic>in vitro</italic> against acetylcholinesterase (AChE) (IC<sub>50</sub> &gt; 100 μM), four different types of human-derived cancer cell lines (IC<sub>50</sub> &gt; 50 μM), as well as methicillin-resistant <italic>Staphylococcus aureus</italic> and <italic>Escherichia coli</italic> (MIC &gt; 50 μg/mL), and <italic>Plasmodium falciparum</italic> W2 (EC<sub>50</sub> &gt; 100 μg/mL), in phenotypic tests.</p>