AUTHOR=Araiza-Villanueva Minerva , Avila-Calderón Eric Daniel , Flores-Romo Leopoldo , Calderón-Amador Juana , Sriranganathan Nammalwar , Qublan Hamzeh Al , Witonsky Sharon , Aguilera-Arreola Ma. Guadalupe , Ruiz-Palma María del Socorro , Ruiz Enrico A. , Suárez-Güemes Francisco , Gómez-Lunar Zulema , Contreras-Rodríguez Araceli 

TITLE=Proteomic Analysis of Membrane Blebs of Brucella abortus 2308 and RB51 and Their Evaluation as an Acellular Vaccine

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02714

DOI=10.3389/fmicb.2019.02714

ISSN=1664-302X

ABSTRACT=<p>Membrane blebs are released from Gram-negative bacteria, however, little is known about <italic>Brucella</italic> blebs. This work pursued two objectives, the first was to determine and identify the proteins in the membrane blebs by proteomics and <italic>in silico</italic> analysis. The second aim was to evaluate the use of membrane blebs of <italic>Brucella abortus</italic> 2308 and <italic>B. abortus</italic> RB51 as an acellular vaccine <italic>in vivo</italic> and <italic>in vitro</italic>. To achieve these aims, membrane blebs from <italic>B. abortus</italic> 2308 and RB51 were obtained and then analyzed by liquid chromatography coupled to mass spectrometry. <italic>Brucella</italic> membrane blebs were used as a “vaccine” to induce an immune response in BALB/c mice, using the strain <italic>B. abortus</italic> RB51 as a positive vaccine control. After subsequent challenge with <italic>B. abortus</italic> 2308, CFUs in spleens were determined; and immunoglobulins IgG1 and IgG2a were measured in murine serum by ELISA. Also, activation and costimulatory molecules induced by membrane blebs were analyzed in splenocytes by flow cytometry. Two hundred and twenty eight proteins were identified in 2308 membrane blebs and 171 in RB51 blebs, some of them are well-known <italic>Brucella</italic> immunogens such as SodC, Omp2b, Omp2a, Omp10, Omp16, and Omp19. Mice immunized with membrane blebs from rough or smooth <italic>B. abortus</italic> induced similar protective immune responses as well as the vaccine <italic>B. abortus</italic> RB51 after the challenge with virulent strain <italic>B. abortus</italic> 2308 (<italic>P</italic> &lt; 0.05). The levels of IgG2a in mice vaccinated with 2308 membrane blebs were higher than those vaccinated with RB51 membrane blebs or <italic>B. abortus</italic> RB51 post-boosting. Moreover, mice immunized with 2308 blebs increased the percentage of activated B cells (CD19<sup>+</sup>CD69<sup>+</sup>) <italic>in vitro.</italic> Therefore, membrane blebs are potential candidates for the development of an acellular vaccine against brucellosis, especially those derived from the rough strains so that serological diagnostic is not affected.</p>