AUTHOR=Govaert Marlies , Smet Cindy , Verheyen Davy , Walsh James L. , Van Impe Jan F. M. 

TITLE=Combined Effect of Cold Atmospheric Plasma and Hydrogen Peroxide Treatment on Mature Listeria monocytogenes and Salmonella Typhimurium Biofilms

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02674

DOI=10.3389/fmicb.2019.02674

ISSN=1664-302X

ABSTRACT=<p>Cold Atmospheric Plasma (CAP) is a promising novel method for biofilm inactivation as log-reduction values up to 4.0 log<sub>10</sub> (CFU/cm<sup>2</sup>) have been reported. Nevertheless, as the efficacy of CAP itself is not sufficient for complete inactivation of mature biofilms, the hurdle technology could be applied in order to obtain higher combined efficacies. In this study, CAP treatment was combined with a mild hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) treatment for disinfection of 1 and 7 day(s) old <italic>Listeria monocytogenes</italic> and <italic>Salmonella</italic> Typhimurium biofilms. Three different treatment sequences were investigated in order to determine the most effective treatment sequence, i.e., (i) first CAP, then H<sub>2</sub>O<sub>2</sub>, (ii) first H<sub>2</sub>O<sub>2</sub>, then CAP, and (iii) a simultaneous treatment of CAP and H<sub>2</sub>O<sub>2</sub>. Removal of the biofilm, induction of sub-lethal injury, and H<sub>2</sub>O<sub>2</sub> breakdown due to the presence of catalase within the biofilms were investigated in order to comment on their possible contribution to the combined inactivation efficacy. Results indicated that the preferred treatment sequence was dependent on the biofilm forming species, biofilm age, and applied H<sub>2</sub>O<sub>2</sub> concentration [0.05 or 0.20% (v/v)]. At the lowest H<sub>2</sub>O<sub>2</sub> concentration, the highest log-reductions were generally observed if the CAP treatment was preceded by the H<sub>2</sub>O<sub>2</sub> treatment, while at the highest H<sub>2</sub>O<sub>2</sub> concentration, the opposite sequence (first CAP, then H<sub>2</sub>O<sub>2</sub>) proved to be more effective. Induction of sub-lethal injury contributed to the combined bactericidal effect, while the presence of catalase within the biofilms resulted in an increased resistance. In addition, high log-reductions were partially the result of biofilm removal. The highest overall log-reductions [i.e., up to 5.42 ± 0.33 log<sub>10</sub> (CFU/cm<sup>2</sup>)] were obtained at the highest H<sub>2</sub>O<sub>2</sub> concentration if CAP treatment was followed by H<sub>2</sub>O<sub>2</sub> treatment. As this resulted in almost complete inactivation of the <italic>L. monocytogenes</italic> and <italic>S.</italic> Typhimurium biofilms, the combined treatment of CAP and H<sub>2</sub>O<sub>2</sub> proved to be a promising method for disinfection of abiotic surfaces.</p>