AUTHOR=Li Bin , Lv Ran , Xiao Ying , Hu Wei , Mai Yuliang , Zhang Jingwen , Lin Lan , Hu Xiaoyong 

TITLE=A Novel Nitrite-Base Aerobic Denitrifying Bacterium Acinetobacter sp. YT03 and Its Transcriptome Analysis

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02580

DOI=10.3389/fmicb.2019.02580

ISSN=1664-302X

ABSTRACT=<p>Nitrite in a water environment is very harmful to humans and aquatic animals. A novel aerobic denitrifying bacterium able to utilize <inline-formula><mml:math id="INEQ1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:msubsup><mml:mtext>NO</mml:mtext><mml:mn>2</mml:mn><mml:mo>-</mml:mo></mml:msubsup></mml:math></inline-formula>-N as the only nitrogen source was isolated for the purpose of removing nitrite from water, which was identified as <italic>Acinetobacter</italic> sp. and named as YT03. The growth and denitrification activity of strain YT03 was assessed comprehensively. Results showed that the nitrite in water with an initial concentration of 10 mg L<sup>–1</sup> could be completely removed within 6 h by strain YT03, and the optimal conditions for strain YT03 to remove nitrite were as follows: sodium succinate as the carbon source, C/N ratio of 16, pH of 6.5, temperature of 30°C, and shaking speed of 250 rpm. An RNA-Seq transcriptome analysis was used to find genes associated with nitrite removal. Compared with the removal of ammonia nitrogen, 47 genes were significantly differentially expressed, including 20 up-regulated and 27 down-regulated genes, mainly involved in the transport process, biosynthetic process, and so on. And among the differentially expressed genes, C4-dicarboxylate transporter (<italic>Dct</italic>A) and nitrate/nitrite transporter (<italic>Nrt</italic>) might be of importance for the efficient utilization of carbon and nitrogen sources in aerobic nitrite denitrification with sodium succinate by strain YT03.</p>