AUTHOR=de Souza Pinto Lemgruber Renato , Valgepea Kaspar , Gonzalez Garcia Ricardo Axayacatl , de Bakker Christopher , Palfreyman Robin William , Tappel Ryan , Köpke Michael , Simpson Séan Dennis , Nielsen Lars Keld , Marcellin Esteban TITLE=A TetR-Family Protein (CAETHG_0459) Activates Transcription From a New Promoter Motif Associated With Essential Genes for Autotrophic Growth in Acetogens JOURNAL=Frontiers in Microbiology VOLUME=10 YEAR=2019 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02549 DOI=10.3389/fmicb.2019.02549 ISSN=1664-302X ABSTRACT=
Acetogens can fix carbon (CO or CO2) into acetyl-CoA via the Wood–Ljungdahl pathway (WLP) that also makes them attractive cell factories for the production of fuels and chemicals from waste feedstocks. Although most biochemical details of the WLP are well understood and systems-level characterization of acetogen metabolism has recently improved, key transcriptional features such as promoter motifs and transcriptional regulators are still unknown in acetogens. Here, we use differential RNA-sequencing to identify a previously undescribed promoter motif associated with essential genes for autotrophic growth of the model-acetogen