AUTHOR=Li Qian , Fan Jia , Sun JingXuan , Zhang Yong , Hou MaoLin , Chen JuLian 

TITLE=Anti-plant Defense Response Strategies Mediated by the Secondary Symbiont Hamiltonella defensa in the Wheat Aphid Sitobion miscanthi

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02419

DOI=10.3389/fmicb.2019.02419

ISSN=1664-302X

ABSTRACT=<p>Bacterial symbionts are omnipresent in insects, particularly aphids, and often exert important effects on the host ecology; however, examples of symbionts that mediate herbivore-plant interactions remain limited. Here, three clones with identical genetic backgrounds were established: a <italic>Hamiltonella defensa</italic>-free clone, <italic>H. defensa-</italic>infected clone and <italic>H. defensa</italic>-cured clone. <italic>H. defensa</italic> infection was found to increase the fitness of <italic>Sitobion miscanthi</italic> by increasing the total number of offspring and decreasing the age of first reproduction. Furthermore, gene expression studies and phytohormone measurement showed that feeding by the <italic>Hamiltonella</italic>-infected clone suppressed the salicylic acid (SA)- and jasmonic acid (JA)-related defense pathways and SA/JA accumulation in wheat plants relative to feeding by the other two clones. Additionally, after feeding by the <italic>Hamiltonella</italic>-infected clone, the activity levels of the defense-related enzymes polyphenol oxidase (PPO) and peroxidase (POD) in wheat plants were significantly decreased compared with the levels observed after feeding by the other two clones. Taken together, these data reveal for the first time the potential role of <italic>H. defensa</italic> of <italic>S. miscanthi</italic> in mediating the anti-plant defense responses of aphids.</p>