AUTHOR=Mendes Lucas William , de Chaves Miriam Gonçalves , Fonseca Mariley de Cassia , Mendes Rodrigo , Raaijmakers Jos M. , Tsai Siu Mui 

TITLE=Resistance Breeding of Common Bean Shapes the Physiology of the Rhizosphere Microbiome

JOURNAL=Frontiers in Microbiology

VOLUME=Volume 10 - 2019

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02252

DOI=10.3389/fmicb.2019.02252

ISSN=1664-302X

ABSTRACT=<p>The taxonomically diverse rhizosphere microbiome contributes to plant nutrition, growth and health, including protection against soil-borne pathogens. We previously showed that breeding for <italic>Fusarium</italic>-resistance in common bean changed the rhizosphere microbiome composition and functioning. Here, we assessed the impact of <italic>Fusarium</italic>-resistance breeding in common bean on microbiome physiology. Combined with metatranscriptome data, community-level physiological profiling by Biolog EcoPlate analyses revealed that the rhizosphere microbiome of the <italic>Fusarium</italic>-resistant accession was distinctly different from that of the <italic>Fusarium</italic>-susceptible accession, with higher consumption of amino acids and amines, higher metabolism of xylanase and sialidase, and higher expression of genes associated with nitrogen, phosphorus and iron metabolism. The resistome analysis indicates higher expression of soxR, which is involved in protecting bacteria against oxidative stress induced by a pathogen invasion. These results further support our hypothesis that breeding for resistance has unintentionally shaped the assembly and activity of the rhizobacterial community toward a higher abundance of specific rhizosphere competent bacterial taxa that can provide complementary protection against fungal root infections.</p>