Fei Liu1Qingyang Liu1Chunyun Yu1Yan Zhao1Yudi Wu1Hui Min1,2Yue Qiu3Ying Jin4Jun Miao2
Liwang Cui2*Yaming Cao1*- 1Department of Immunology, College of Basic Medical Sciences, China Medical University, Shenyang, China
- 2Department of Internal Medicine, Morsani College of Medicine, University of South Florida, Tampa, Tampa, FL, United States
- 3The First Hospital of China Medical University, Shenyang, China
- 4Liaoning Research Institute of Family Planning, Shenyang, China
Sexual reproduction is an essential process in the Plasmodium life cycle and a vulnerable step for blocking transmission from the human host to mosquitoes. In this study, we characterized the functions of a conserved cell membrane protein P115 in the rodent malaria parasite Plasmodium berghei ANKA. Pb115 was expressed in both asexual stages (schizonts) and sexual stages (gametocytes, gametes, and ookinetes), and was localized on the plasma membrane of gametes and ookinetes. In P. berghei, genetic deletion of Pb115 (Δpb115) did not affect asexual multiplication, nor did it affect gametocyte development or exflagellation of the male gametocytes. However, mosquitoes fed on Δpb115-infected mice showed 74% reduction in the prevalence of infection and 96.5% reduction in oocyst density compared to those fed on wild-type P. berghei-infected mice. The Δpb115 parasites showed significant defects in the interactions between the male and female gametes, and as a result, very few zygotes were formed in ookinete cultures. Cross fertilization with the male-defective Δpbs48/45 line and the female-defective Δpfs47 line further indicated that the fertilization defects of the Δpb115 lines were present in both male and female gametes. We evaluated the transmission-blocking potential of Pb115 by immunization of mice with a recombinant Pb115 fragment. In vivo mosquito feeding assay showed Pb115 immunization conferred modest, but significant transmission reducing activity with 44% reduction in infection prevalence and 39% reduction in oocyst density. Our results described functional characterization of a conserved membrane protein as a fertility factor in Plasmodium and demonstrated transmission-blocking potential of this antigen.
Introduction
Malarial incidence has significantly decreased in recent years due to a range of actions, including the deployment of insecticide-treated nets, indoor residual spraying and artemisinin-based combination therapies (Bhatt et al., 2015). Nevertheless, recent World Health Organization reports showed that the global progress toward malaria elimination has stalled (World Health Organization [WHO], 2018). To achieve global elimination of malaria, an integrated malaria control strategy and novel interventions are needed, which may include measures that interrupt and inhibit disease transmission.
During the complex life cycle of malaria parasite, sexual stages are obligative for the transmission of the parasite from the human host to the mosquitoes. Male and female gametocytes formed in the human blood, after ingestion by a female anopheline mosquito, undergo gametogenesis to form gametes, which then fuse to form zygotes. Later, zygotes mature into ookinetes, which penetrate the midgut epithelium to differentiate into oocysts.
Transmission from the human host to the mosquito represents a major population bottleneck in the life cycle of the parasite (Vaughan, 2007). Transmission-blocking vaccines (TBVs) target this bottleneck by eliciting antibodies that inhibit the fusion of the male and female gametes (pre-fertilization) and maturation from zygotes to ookinetes (post-fertilization). To date, a substantial number of antigens expressed during sexual development have been studied for their TBV potentials (Nikolaeva et al., 2015; Wu et al., 2015; Delves et al., 2018), but only three antigens, namely the pre-fertilization antigens Pfs48/45 (Kocken et al., 1993; Outchkourov et al., 2008; Chowdhury et al., 2009) and Pfs230 (Williamson et al., 1993; Lee et al., 2017; Tachibana et al., 2019), as well as the post-fertilization antigen Pfs25 (Kaslow et al., 1988; Shimp et al., 2013; Talaat et al., 2016; Sagara et al., 2018) and its ortholog Pvs25 in Plasmodium vivax (Tsuboi et al., 1998; Sagara et al., 2018), have been extensively investigated as lead vaccine candidates. However, all these TBV candidates are conformational antigens containing multiple disulfide bridges, and the production of correctly folded antigens is an important challenge (Sauerwein and Bousema, 2015). Therefore, continuous efforts on TBV antigen discovery are warranted.
The deciphering of Plasmodium genomes has provided an unprecedented opportunity for large-scale functional studies toward a better understanding of the fundamental developmental biology of the parasite (Gardner et al., 2002; Hall et al., 2005). This has also fueled the functional screening of potential TBV antigens during ookinete development in the more genetically amenable rodent parasite Plasmodium berghei (Ecker et al., 2008). Using a similar strategy, we identified Pb115, an evolutionarily conserved, putative membrane protein that is expressed in both asexual and sexual stages of the malaria parasites. Through genetic manipulation studies in P. berghei, we found that the mutant parasites lacking Pb115 had a major defect in ookinete formation, resulting in transmission failure to the mosquitoes. Genetic crosses revealed that both male and female gametes require this protein for gamete recognition and attachment. Immunization of mice with the recombinant Pb115 protein induced strong antibody responses that effectively blocked formation of ookinetes, highlighting the TBV potential of this protein.
Materials and Methods
Mice, Parasites, and Mosquitoes
Six-to-eight-week old female BALB/c mice were purchased from Beijing Animal Institute (Beijing, China). The P. berghei ANKA strain 2.34 was maintained by serial passage and used for challenge infections as described previously (Blagborough and Sinden, 2009). Adult female Anopheles stephensi mosquitoes (Hor strain) were reared in an insectary under 25°C, 50–80% humidity and a 12 h light/dark cycle, and fed 10% (w/v) glucose solution-soaked cotton balls. All animal experiments were approved by the animal ethics committee of China Medical University.
Sequence Analysis
To identify genes encoding potential ookinete surface proteins, we searched the malaria database PlasmoDB1 for proteins expressed in ookinetes with a putative signal peptide or at least one transmembrane domain. We identified a gene PBANKA_0931000, which encodes a putative protein of 115 kDa, henceforth designated as Pb115. The sequences of its orthologs in other Plasmodium species were retrieved from PlasmoDB and aligned using the ClustalW multiple sequence alignment program.
Generation of Transgenic Parasites
The HA-tagging and gene-knockout (KO) targeting vectors for Pb115 (PbGEM-290856 and PbGEM-290848) were acquired from PlasmoGEM (Wellcome Trust Sanger Institute, Cambridge, United Kingdom). HA-tagged (pb115-HA) and Pb115 KO (Δpb115) parasite lines were generated by homologous recombination as previously described (Braks et al., 2006). To obtain schizonts for transfection, parasitized red blood cells (RBCs) were collected from mice at day 4 after infection and cultured at 37°C overnight in the culture medium [RPMI 1640, 20% (v/v) fetal calf serum, and 50 mg/L penicillin and streptomycin] at 0.5 mL blood/50 mL medium. The culture was then fractionated on a 55% (v/v) Nycodenz cushion to purify the schizonts (Dempsey et al., 2013). Linearized plasmids were transfected into the schizonts using Nucleofector II (Lonza, Stockholm, Sweden), and the parasite suspension was intravenously injected into two BALB/c mice through the tail vein. At 24 h after injection, mice were treated with pyrimethamine (0.07 mg/ml) via drinking water for 3–4 days. PCR was used to identify the desired integration events in transfected parasites using integration-specific primers (Supplementary Table S1). For tagging, the HA tag was inserted at the C-terminus of the pb115 gene, and for KO, the open reading frame of pb115 was replaced with an hdhfr expression cassette. Parasites were cloned by limiting dilution. Primers for 5′ or 3′ recombination fragments and integration-specific PCR are listed in Supplementary Table S1.
Expression of Recombinant Pb115 (rPb115) and Immunization
A 205 amino acid (aa) fragment of the Pb115 (aa 756–960) was expressed in the yeast Pichia pastoris. Briefly, the Pb115 DNA fragment was amplified using primers CGTACGTACAAAATATTAAAACTATTTTTTCATCATTT and TTGCGGCCGCTCTTGCCTTCCAATATATGGTAAATATTAG (restriction sites underlined) and cloned into the pPIC3.5K(+His) plasmid. A positive yeast strain was cultured in 1 L of BMMG medium and rPb115 expression was induced with methanol. Yeast cells were collected by centrifugation and lysed using an ATS high pressure homogenizer (ATS Engineering Inc., Germany). Recombinant proteins were purified with the Ni-NTA column (Novagen, Germany). The quality of purified protein was analyzed by SDS-PAGE.
To generate antisera against Pb115, 6–8-week old female BALB/c mice (n = 5) were immunized with 50 μg of purified rPb115 emulsified with complete Freund’s adjuvant. Mice were then given two booster injections at 2-week intervals with 25 μg of protein, each emulsified with incomplete Freund’s adjuvant. Mice in the control group (n = 5) were immunized with adjuvant formulations in phosphate buffered saline (PBS, pH 7.0). Two weeks after the final immunization, blood was collected from mice via cardiac puncture and allowed to clot at room temperature to obtain the antisera. An enzyme-linked immunoassay (ELISA) was used to analyze the antibody titers as previously described (Chan et al., 2014).
Western Blot
Western blots were performed with parasite lysates and protein fractions of different developmental stages. Purified schizonts, gametocytes, and ookinetes were treated with 0.15% saponin (Sigma) in PBS for 10 min on ice. Parasites were collected by centrifugation and washed once with PBS. Parasite proteins were collected following repeated extraction in PBS containing 1% Triton X-100, 2% SDS and protease inhibitors (Roche, Basel, Switzerland) for 30 min at room temperature. Lysates of non-infected erythrocytes and Δpb115 ookinetes were used as negative controls. For subcellular protein fractionation, plasma membrane (PM) and cytoplasm of the parasites were separated using the MinuteTM Plasma Membrane Protein Isolation and Cell Fractionation Kit (Invent Biotechnologies Inc., United States) according to the manufacturer’s instructions. Protein concentration was determined using the BCA method, and equal amounts of protein lysates or cellular fractions (10 μg) were separated in 6% SDS-PAGE gels. For Western blots, proteins were transferred to a 0.22 μm polyvinylidene difluoride membrane (Bio-Rad, Hercules, CA, United States). The membrane was blocked with 5% non-fat milk in TBS buffer containing 0.1% Tween 20 (TBST) for 2 h, and then probed with anti-rPb115 antisera at 1:1000 or the anti-HA monoclonal antibody (mAb) at 1:1000 (Invitrogen, Carlsbad, CA, United States) for 2 h. Mouse anti-PbHsp70 antiserum (1:1000) was used as a control to estimate protein loading. After washing three times with TBST, bound primary antibodies were detected with HRP-conjugated goat anti-mouse antibodies (Invitrogen) diluted 1:10,000 in TBST. After three washes with TBST, proteins on the blot were visualized using a Pierce ECL Western Blotting Kit (Thermo Fisher Scientific). Band intensities were measured by ImageJ and the relative expression levels of Pb115 protein were normalized against those of PbHsp70 for the corresponding stages.
Indirect Immunofluorescence Assay
For Indirect Immunofluorescence Assay (IFA), HA-tagged or wild-type (WT) parasites were fixed with 4% paraformaldehyde and 0.0075% glutaraldehyde in PBS for 20 min at room temperature and rinsed with 50 mM glycine in PBS. To differentiate internal and external protein localizations, cells were either permeabilized with 0.1% Triton X-100/PBS for 10 min or directly processed without permeabilization. To liberate schizonts and gametocytes from the enveloping RBC and parasitophorous vacuole membranes, schizonts and gametocytes were treated with 0.05% saponin/PBS for 3 min at 37°C prior to fixation. Without further membrane permeabilization, cells were washed in PBS and then treated with 0.1 mg/ml of sodium borohydride in PBS for 10 min to reduce the free aldehyde groups (Tonkin et al., 2004). After blocking with PBS containing 3% BSA/PBS at 37°C for 1 h, WT and Pb115-HA parasites were incubated with mouse anti-rPb115 antisera (1:500) and mouse anti-HA mAb (1:500, Invitrogen), respectively, at 37°C for 1 h. Cells were co-incubated with rabbit antisera against PbMSP1, Pbg377, α-tubulin and PSOP25 as stage-specific markers for schizonts, female gametocytes/gametes, male gametocytes/gametes, and ookinetes, respectively. Then the slides were washed three times with PBS and incubated with polyclonal Alexa Flour 488-conjugated goat-anti-mouse IgG secondary antibodies (1:500, Invitrogen) and Alexa Flour 555-conjugated goat-anti-rabbit IgG secondary antibodies (1:500, Cell signaling), respectively, at 37°C for 30 min. Parasite nuclei were stained with 4, 6-diamidino-2-phenylindole (DAPI, Invitrogen) at a final concentration of 1 μg/mL. As negative controls, WT ookinete smears were incubated mouse anti-HA mAb (1:1000, Invitrogen) and control sera obtained from mice immunized with adjuvant emulsified in PBS as primary antibodies, or secondary antibodies alone. Slides were mounted with ProLong® Gold antifade reagent (Invitrogen) and observed under a Nikon C2 fluorescence confocal laser scanning microscope.
Phenotypic Analysis of Δpb115
To study the functions of Pb115 during the Plasmodium development, mice in each group were injected with either 1 × 106 WT P. berghei- or Δpb115-infected RBCs (iRBCs) (Clone 1 and Clone 2). Parasitemia and mortality of mice were monitored daily. To determine the effect on parasite sexual development, mice were pre-treated with 0.2 mL of 6 mg/mL phenylhydrazine for 3 days before injection of iRBCs. On day 3 after infection, gametocytemia and the gametocyte sex ratio were determined by Giemsa-stained tail blood smears (Guttery et al., 2014). At least 100 mature gametocytes were quantified as males and females to determine the gametocyte sex ratio (Liu et al., 2018). Exflagellation centers of male gametocytes and male–female gametes interactions were quantified as previously described (Tewari et al., 2010). In short, 10 μL tail blood from infected mice was added to 40 μL standard ookinete medium (RPMI 1640 with 50 mg/L penicillin, 50 mg/L streptomycin, 100 mg/L neomycin, 20% [v/v] heat-inactivated fetal calf serum [FCS], pH 8.0). Fifteen minutes after induction of gamete formation at 25°C, 1 μL of culture was placed on a coverslip (Matsunami Glass Ind., Ltd., Japan) and analyzed under a light microscope (40× objective). The exflagellation centers were counted as an exflagellating male gametocyte with four red blood cells in 10 min. Male–female gametes interactions were counted as the males attached females for more than 3 s during a period of 20 min in 10 fields (40× objective) (van Dijk et al., 2010). To count the macrogamete numbers, ookinete culture was set up as described above. After incubation at 25°C for 15 min, 1 μL of culture were placed on a coverslip and female gametes were stained with mouse anti-Pbs21 antibody (1:1000) without permeabilization. The numbers of female gametes in 1 μL of culture were counted under a fluorescence microscope (100× oil objective). The culture was further incubated at 19°C; 1 μL of culture was taken out at 2 h and 24 h to determine the number of zygotes and ookinetes, respectively (Tewari et al., 2010). To determine whether defects of Δpb115 lines were female- or male-specific, Pbs47 KO (Δpbs47) and Pbs48/45 KO (Δpbs48/45) lines were used in an in vitro cross-fertilization assay as described (van Dijk et al., 2010; Zhu et al., 2019). At 3 days post infection, equal numbers of mature gametocytes of different clones were mixed and incubated for 24 h. The numbers of ookinetes were counted as described above (100× oil objective).
To determine the subsequent development, mosquito feeding experiment was performed with mice that were infected three days earlier with either the WT or Δpb115 P. berghei. Four-day-old female A. stephensi mosquitoes (∼100/mouse) starved for 6 h were allowed to feed on infected mice for 30 min. Unfed mosquitoes were then removed and fed mosquitoes were maintained at 19–22°C and in 50–80% relative humidity. Ten days after feeding, up to 50 mosquitoes were dissected in each group. The midguts of mosquitoes were removed and stained with 0.5% mercurochrome (Sigma-Aldrich). Oocysts were counted to determine the prevalence (number of infected mosquitoes) and intensity of infection (number of oocysts per positive midgut).
Quantification of Transmission-Blocking Activity
Transmission-blocking activity (TBA) of anti-rPb115 antisera was estimated using both in vitro ookinete conversion and in vivo mosquito feeding assays (Kou et al., 2016). In short, phenylhydrazine pre-treated mice were injected with 1 × 106 WT iRBCs. Three days post-infection, parasitemia was counted by Giemsa staining, and 10 μL gametocyte-infected blood were mixed with 90 μL ookinete culture medium containing 10% anti-Pb115 mouse sera or control sera (immunized with adjuvants only) incubated at 19°C for 2 h to count zygote numbers and 24 h to count ookinete numbers. Mosquito-feeding experiments were carried out as described previously (Zheng et al., 2017). Immunized mice with rPb115 protein or control mice were pre-treated with 0.2 mL of 6 mg/mL phenylhydrazine for 3 days. Five mice from each group were injected with 1 × 106 WT iRBCs, and mosquitoes were fed on immunized mice at day 3 after infection. Ten days after blood meal, the prevalence and intensity of infection in mosquitoes were determined.
Statistical Analysis
Statistical comparison between groups (IgG levels, parasitemia, gametocytemia, and ookinete numbers) was performed by Student’s t test using the GraphPad Prism software. The intensity of mosquito infection (oocysts/midgut) was analyzed using the Mann–Whitney U test, while infection prevalence was analyzed by Fisher’s exact test using SPSS version 21.0. Survival of mice infected with WT or Δpb115 parasites was compared by using the Kaplan–Meyer’s method. All data were from three independent experiments.
Results
P115 Is Highly Conserved Among Plasmodium Species
PBANKA_0931000 (Pb115) is among a group of genes with the following features: conserved in Plasmodium genomes; sexual-stage expression; and containing the sequence for a putative signal peptide or at least one transmembrane domain (Zheng et al., 2016). The Pb115 gene is located on chromosome 9 and encodes a protein of 978 aa with a predicted size of 115 kDa. As shown in the predicted protein features in PlasmoDB, the predicted protein contains a membrane lipoprotein lipid attachment site at the N-terminus and a major facilitator superfamily (MFS) general substrate transporter domain (P value 1.05e-14) near the C terminus (Supplementary Figure S1). The encoded protein contains 12 transmembrane domains, six within the MFS domain. Multiple sequence alignment showed that this protein is highly conserved among Plasmodium species (Supplementary Figure S2).
Pb115 Is Expressed in Both Asexual and Sexual Stages
Expression of the ortholog of Pb115 in Plasmodium falciparum (PF3D7_1117000), as demonstrated by RNA-seq analysis, was mainly in schizonts during the asexual intraerythrocytic development cycle (Plasmodb.org). The PF3D7_1117000 mRNA was also detected during sexual development and was almost equally abundant in male and female gametocytes (Lasonder et al., 2016). To study Pb115 protein expression during P. berghei development, we generated a recombinant Pb115 fragment, which was used to generate antibodies directed against the protein. For this, the 205 aa MFS domain from aa 756 to aa 960 (Supplementary Figure S1) was expressed in yeast and purified using the Ni-NTA column. SDS-PAGE of the purified recombinant protein (rPb115) showed a relatively homogenous protein band with a molecular weight of ∼23 kDa (Figure 1A), which agreed with the predicted size of the MFS domain. The rPb115 protein was used to immunize 6–8-week-old female BALB/c mice. Two weeks after the third immunization, the immune sera contained a significantly higher antibody titer against the rPb115 fragment than the adjuvant control sera (Supplementary Figure S3). The antisera were used to probe lysates from purified P. berghei schizonts, gametocytes and cultured ookinetes in Western blots. Compared with the erythrocyte lysate control, the anti-rPb115 antisera specifically recognized a protein of approximately 115 kDa in the three developmental stages, consistent with the predicted molecular size of Pb115. Using HSP70 for protein loading control, Pb115 showed similar expression levels in all these three stages examined (Figure 1B). ImageJ analysis of the band intensities in three replicated Western blots showed a slight lower Pb115 abundance in ookinetes than in schizonts and gametocytes (Figure 1C).
Figure 1. Recombinant Pb115 protein purification and detection of Pb115 expression during parasite development. (A) Purified recombinant Pb115 protein was subjected to electrophoresis on a 12% polyacrylamide gel, and the proteins were stained with Coomassie Brilliant Blue. M: PageRuler Prestained Protein Ladder in kDa. (B) Representative Western blot of Pb115 in asexual- and sexual-stage parasites. Lysates of schizonts (Sch), gametocytes (Gam), ookinetes (Ook), and Δpb115 parasites (KO) at 10 μg/lane were probed with mouse anti-Pb115 sera (1:500). The Δpb115 parasite protein extract was a mixture of 7 μg asexual-stage proteins and 3 μg ookinete proteins. Protein loading was estimated by Western blot with the anti-Hsp70 sera (1:1000). Non-infected erythrocytes (EC) were used as negative control. (C) The Pb115 relative protein levels in schizonts (Sch), gametocytes (Gam), ookinetes (Ook), and KO parasites were estimated by using ImageJ analysis of replicate Western blots shown in (B). Data were normalized against the PbHsp70 bands of the respective stages.
To further verify that the anti-rPb115 sera indeed identified the Pb115 protein, we generated a P. berghei parasite line with the endogenous Pb115 tagged with HA at its C-terminus. Correct fusion of the Pb115 gene with the HA tag was confirmed by diagnostic integration PCR (Supplementary Figure S4). Western blotting with the anti-HA mAb identified a protein band of ∼115 kDa, similar to the size of the protein identified by the anti-Pb115 antisera. No specific protein bands were detected in lysates from non-infected erythrocytes and WT ookinetes (Supplementary Figure S4). Similarly, Pb115-HA protein level in ookinetes was slightly lower than those in schizonts and gametocytes.
Pb115 Is Localized on the Plasma Membrane of Gametes and Ookinetes
The presence of multiple transmembrane domains in Pb115 suggests that this protein might be associated with membrane structures in the parasites. To detect membrane association of Pb115, we separated the parasite PM with the cytoplasm and performed Western blots using the anti-Pb115 antisera. In schizonts and gametocytes, Pb115 was detected in both the cytoplasm and PM fractions, with Pb115 appearing more abundant in the PM fractions (Supplementary Figure S5). Interestingly, in ookinetes, Pb115 was mainly detected in the PM fraction. Using the same procedure, we evaluated the subcellular distribution of Pb115 in the Pb115-HA parasite line using the anti-HA mAb. The results obtained using both the WT and Pb115-HA lines were highly comparable (Supplementary Figure S5).
We further examined Pb115 expression and localization in more detail by IFA. In WT parasites, IFA with the anti-rPb115 sera detected Pb115 protein expression in schizonts, gametocytes, gametes, and ookinetes (Figure 2), whereas fluorescence was almost undetectable in rings, trophozoites and sporozoites (data not shown). As negative controls, IFA with control mouse sera or without primary antibodies did not produce fluorescence signals (Figure 2). In both schizonts and gametocytes, Pb115 fluorescence dispersed throughout the cytoplasm, sometimes with a speckled appearance. In female gametes, the Pb115 signal was found to be associated with the PM only, whereas in male gametes, it was associated with both the flagella and the residual body (Figure 2). Consistent with the Western analysis showing primary association of Pb115 with the PM in ookinetes, IFA also showed association of Pb115 with the PM of ookinetes with the signals partially being co-localized with those of PSOP25. To differentiate internal from external membrane localization, IFA was performed without membrane permeabilization. In this case, schizonts and gametocytes were not labeled, indicating that the Pb115 protein was not localized outside of the membrane of the iRBC (Figure 2). During gamete development, the female gametes showed a similar localization pattern regardless of membrane permeabilization status, demonstrating external membrane localization of the Pb115 on female gametes. Similarly, in male gametes, the fluorescent signals were detected on both residual bodies and flagella-like male gametes. Again, Pb115 was clearly localized on the PM of ookinetes (Figure 2). IFA with developmental stages of the Pb115-HA line using anti-HA mAb showed highly similar patterns of Pb115 localization (Supplementary Figure S6).
Figure 2. Representative IFA images of Pb115 in WT P. berghei with (+TX) or without (–TX) membrane permeabilization. Parasites were incubated with anti-Pb115 sera (1: 500) as the primary antibodies (green). The parasites were also labeled with antibodies against the marker proteins for different stages (PbMSP1 for schizonts, Pbg377 for female gametocytes and gametes, α-tubulin for male gametocytes and gametes, and PSOP25 for ookinetes). Nuclei were stained with DAPI (1 μg/mL) (blue). The bottom panel shows two negative controls: WT ookinetes (+TX) labeled with the control serum or only with the secondary antibodies (AF488, Alexa Fluor 488, and AF555, Alexa Fluor 555). Merge, Alexa Flour 488 + Alexa Flour 555 + DAPI. Bar, 5 μm. Data are representatives of three independent experiments.
To further demonstrate the PM association of Pb115 in schizonts and gametocytes, parasites were liberated from the enveloping RBC and parasitophorous vacuole membranes via mild saponin treatment. Subsequent IFA showed that Pb115 was localized at the peripheral PM in both male and female gametocytes (Supplementary Figure S7A). This localization pattern was further confirmed using the Pb115-HA line probed with the anti-HA mAb (Supplementary Figure S7B). Collectively, these data indicated that Pb115 was expressed from schizonts to ookinetes, and Pb115 retained PM localization from gametes through ookinete development. Given that the Pb115 antisera were generated against the C-terminus and HA was also tagged to the C-terminus, these localization patterns suggest that the Pb115 C-terminus was external to the PM.
Pb115 Is Needed for Gamete Attachment
To investigate the function of Pb115 in P. berghei development, the pb115 gene was knocked out by homologous recombination. Integration-specific PCR was used to confirm the KO lines (Supplementary Figures S8A,B). In addition, the lack of Pb115 protein expression in a KO line was confirmed by Western blot analysis (Figures 1B,C) and IFA (Supplementary Figure S8C). Two Δpb115 lines were selected from two independent transfection experiments and used for phenotype analysis. The effect of the pb115 KO on parasite development was examined during blood-stage infection and in mosquitoes. Although Pb115 was expressed in schizonts, deletion of pb115 did not affect asexual blood-stage multiplication of the parasites (P > 0.05; Figure 3A), nor did it impact the survival of infected mice (P > 0.05, Kaplan–Meier’s survival analysis; Figure 3B). With regard to sexual stages, there were no differences in gametocytemia and sex ratio between the WT and Δpb115 lines (P > 0.05; Figures 3C,D). Furthermore, the Δpb115 parasites showed no defects in gametogenesis, as the numbers of exflagellation centers and macrogamete numbers were all comparable between the WT and the Δpb115 lines (P > 0.05; Figures 4A,B). Using in vitro assays, we then evaluated whether pb115 KO affected the fertilization and subsequent sexual development process. During our in vitro culture of ookinetes, the zygote numbers formed at 2 h and ookinete numbers at 24 h in the two Δpb115 clones suffered similar levels of reduction (∼95%) compared to the WT parasites (P < 0.01; Figures 4C,D), suggesting that Δpb115 parasites were defective in fertilization. This finding was further reinforced by results from mosquito feeding experiments, where A. stephensi mosquitoes were allowed to feed on WT P. berghei- or Δpb115-infected mice and midgut oocysts were quantified. Mosquitoes fed on WT P. berghei-infected mice had 88–96% prevalence of infection in three experiments, whereas mosquitoes fed on Δpb115-infected mice had infection prevalence ranging from 12 to 16%, a reduction by 74% (P < 0.001; Table 1). Similarly, a drastic reduction in oocyst density was observed in mosquitoes fed on Δpb115-infected mice (P < 0.001; Table 1 and Supplementary Figure S9A). Specifically, the mean number of oocysts per midgut was 118 in mosquitoes fed on WT-infected mice as compared to <4 on average in those fed on Δpb115-infected mice.
Figure 3. Functional analysis of Pb115 during parasite blood stages. Mice were infected with P. berghei or Δpb115 parasites Clone 1 (C1) and Clone 2 (C2). (A) Growth curve of asexual stages (10 mice per group). (B) Survival rates of mice infected with different parasite strains (10 mice per group). (C) Gametocytemia on day 3 after infection by different parasite strains (five mice per group). (D) Female: male gametocyte ratios on day 3 after infection by WT P. berghei and Δpb115 clone 1 and 2 (five mice per group). The data are from three biological experiments and are presented as the mean ± SD in each group.
Figure 4. Function analysis of Pb115 in sexual stages. P. berghei or Δpb115 parasites (Clone 1 and Clone 2)-infected mouse blood on day 3 after infection was incubated in the ookinete culture medium. (A) Exflagellation centers of male gametocytes in 10 microscopic fields under a 40× objective. (B) Numbers of female gametes in 1 μL of ookinete culture at 15 min post activation (C) Numbers of zygotes formed at 2 h during in vitro ookinete culture. (D) Numbers of ookinetes formed at 24 h during in vitro ookinete culture. Data are presented as the mean ± SD for five mice in each group. (E) Male–female gamete interactions. Numbers of male gametes attached to females for more than 3 s during 20 min of observation. Data are presented as the mean ± SD from three experiments. ∗∗ indicates P < 0.01 for comparison with WT parasite (t test).
Table 1. Phenotypic comparison between Δpb115 and WT P. berghei in mosquitoes.
To detect which step of the fertilization process was defective in the Δpb115 parasites, we performed detailed observations of the male–female gamete interactions under a phase contrast microscope. Male gametes from both WT and Δpb115 parasites exhibited similar motility and interaction with the RBCs, as evidenced by the similar numbers of exflagellation centers formed. In WT parasites, male–female attachments (for >3 s) were readily observed; on average 22 attachments were seen in five experiments (Figure 4E). By contrast, the number of gametes forming male–female attachments in the Δpb115 parasite lines was drastically reduced (five observed in eight experiments) (P < 0.01; Figure 4E), indicating that Δpb115 gametes were defective in recognition and attachment. To determine whether the resultant defects in gamete attachment in Δpb115 were due to either the male or female gamete, we performed in vitro cross-fertilization experiments between Δpb115 gametocytes and Δpbs47 or Δpbs48/45 gametocytes. As found in earlier studies (van Dijk et al., 2010), both Δpbs47 and Δpbs48/45 lines showed a fertilization rate that was decreased by more than 99% compared to WT (Figure 5). Since it has been reported that Δpbs47 produces normal male but defective female gametes, whereas the Δpbs48/45 line produces normal female but defective male gametes, we performed in vitro cross-fertilization to confirm that each line only had defects in one sex. As expected, similar numbers of ookinetes were formed in the Δpbs47 × Δpbs48/45 cross as compared to the WT (Figure 5). However, cross-fertilization of Δpb115 with either Δpbs47 or Δpbs48/45 failed to generate an appreciable number of ookinetes as compared with cross-fertilization between Δpbs47 and Δpbs48/45 (P < 0.01; Figure 5), suggesting that pb115 deletion affected both male and female gametes.
Figure 5. In vitro cross-fertilization studies. Δpb115 clone C1 and C2 and parasite lines that produce only fertile male (Δpbs47) or only fertile female (Δpbs48/45) gametes were incubated in different combinations during in vitro ookinete culture. The numbers of ookinetes formed were counted at 24 h. Data are from three independent experiments. ∗∗ indicates P < 0.01 for the comparison with WT P. berghei (t test).
Antibodies Against Pb115 Display Apparent TRA
Given that Pb115 was found localized on the PM of gametes and ookinetes, we wanted to test whether Pb115 had TRA. In the in vitro ookinete conversion assay, the number of zygotes formed during in vitro ookinete culture with the anti-rPb115 mouse sera was 5.3 times lower than those with the control sera (P < 0.01; Figure 6A). The number of ookinetes formed with the anti-rPb115 mouse sera at 24 h was 5.7 times lower than those with the control sera (P < 0.01; Figure 6B), suggesting that the immune sera had a major effect on reducing fertilization. In mosquito feeding assays, mice were immunized with rPb115 proteins and then infected with the WT P. berghei. Three days post infection, A. stephensi mosquitoes were allowed to directly feed on rPb115-immunized and control mice. Mosquitoes fed on control mice had an infection prevalence of 92–96% and oocyst density of 109.9–132.6 oocysts/midgut (Table 2). In comparison, mosquitoes fed on rPb115-immunized mice had infection prevalence of 44–52%, a reduction of 44% compared to mosquitoes fed on immunization control mice (P < 0.001, Fisher’s exact test, Table 2). Similarly, moderate levels of reduction (39%) in oocyst density (70–80.1 oocysts/midgut) were also observed in mosquitoes fed on rPb115-immunized mice as compared to those fed on control mice (P < 0.001, Mann–Whitney U test, Table 2 and Supplementary Figure S9B).
Figure 6. Quantification of TRA of the anti-Pb115 sera in vitro. Gametocyte-infected blood on day 3 after infection with P. berghei was incubated at 19°C in ookinete culture medium containing 10% anti-Pb115 mouse sera or control sera, and the numbers of zygotes and ookinetes formed at 2 and 24 h were counted. (A) Zygote numbers at 2 h. (B) Ookinete numbers at 24 h. The data are presented as the mean ± SD. ∗∗ indicates P < 0.01 for the comparison with the adjuvant control sera (t test).
Table 2. In vivo evaluation of transmission blocking activity of Pb115.
Discussion
Key fertilization factors discovered during functional studies of Plasmodium gamete membrane proteins include the male fertility factors Pbs48/45 and Pbs230 and female fertility factor Pbs47, which play critical roles in recognition and attachment to gametes of the opposite sex in the rodent parasite P. berghei (van Dijk et al., 2001, 2010). Interestingly, Pfs230 and Pfs47 seem to have divergent functions in P. falciparum: Pfs230 mediates erythrocyte binding (Eksi et al., 2006), whereas Pfs47 is dispensable for female gamete fertility (van Schaijk et al., 2006) but is critical for immune evasion in mosquitoes (Molina-Cruz et al., 2013). Another male fertility factor, HAP2/GCS1, functions after the initial gamete recognition and attachment, since PbHAP2 deletion lines have normal exflagellation and pairing of the male gametes with female gametes, but lack gamete fusion (Hirai et al., 2008; Liu et al., 2008).
In the current study, we identified a new gamete membrane protein, Pb115, whose deletion affected gamete fertility. The Pb115 protein has a conserved C-terminal MFS domain that is found in the MFS of transporter proteins, the largest superfamily of secondary carriers that are ubiquitously found in all branches of life with nearly 15,000 members. MFS transporters move a myriad of small molecules across biological membranes including sugars, peptides, deleterious substances, organic and inorganic ions (Yan, 2015). Based on the transport mode, MFS transporters are classified into uniporters (transporting a single substrate), symporters (transporting a substrate with a co-transporting ion or solute in the same direction), and antiporters (transporting a substrate with a co-transporting substrate in the opposite direction). They play diverse roles in homeostasis, metabolism and signal transduction (Law et al., 2008). It will be interesting to determine what, if any, compounds are transported by Pb115.
We learned from the current study that Pb115 is not essential for the intraerythrocytic stages – schizonts and gametocytes – despite its expression in these stages. In addition, gametogenesis in the absence of Pb115 appeared normal. Although the male gametes in the Δpb115 parasite showed similar motility to the WT parasites, and made contacts with the female gametes, most of the male–female interactions were transient (lasting less than 3 s) and futile. Furthermore, both male and female gametes were similarly affected by pb115 deletion, as cross-fertilization with either Δpbs48/45 or Δpbs47 was not able to restore fertilization in the Δpb115 lines. It is noteworthy that despite the very low fertilization rates in the Δpb115 parasites, sporozoites dissected from salivary glands of Δpb115-infected mosquitoes were equally infective to mice as the WT sporozoites (data not shown), suggesting the defects observed with Δpb115 were probably limited to gamete adhesion. Whether Δpb115 affects transport of essential molecules needed for male and/or female gamete functions or whether P115 impacts distribution of key fertility factors described above remains to be determined.
We have demonstrated using IFA that Pb115 was associated with the plasma membrane of gametes and ookinetes. Furthermore, Pb115 appeared to have a membrane localization conformation with its C-terminal domain residing outside the cell, given that the antibodies raised against MFS could detect Pb115 without membrane permeabilization. In addition, anti-HA mAb also detected the Pb115 C-terminal HA tag on gametes and ookinetes in a similar way. Since several gamete membrane proteins such as P48/45 and P230 are primary TBV candidates, the membrane expression of Pb115 during sexual stages prompted us to investigate its transmission-blocking potential. We generated the recombinant MFS domain from yeast and immunized mice to study its TRA using both in vitro ookinete conversion and in vivo mosquito feeding assays. Antibodies against the synthetic protein could recognize Pb115 in Western blot and IFA, suggesting that the recombinant protein possessed, at least in part, properly folded epitopes. Antisera against rPb115 significantly reduced zygote formation and ookinete conversion. Direct feeding of mosquitoes on rPb115-immunized mice showed 44% reduction in prevalence and 39% reduction in oocyst intensity as compared to those fed on non-immunized mice. Compared with the TRA of other gamete membrane proteins such as P48/45 (Outchkourov et al., 2008), P230 (Williamson et al., 1995), and HAP2 (Blagborough and Sinden, 2009), the extents of reduction are rather modest and further refinement of the recombinant protein covering important epitopes may help improve the TB activity of Pb115 (Deore et al., 2019). The expression of Pb115 on both gametes and ookinetes suggests that it might be a target for both pre- and post-fertilization immunity. In addition, studies on P115 in human malaria parasites are warranted, given the high degree of conservation of P115 in different Plasmodium species.
Data Availability Statement
The raw data supporting the conclusions of this manuscript will be made available by the authors, without undue reservation, to any qualified researcher.
Ethics Statement
This study was carried out in accordance with the recommendations of the guidelines established by China Medical University, Animal Welfare and Research Ethics Committee. The protocol was approved by the Animal Care and Use Committee of China Medical University.
Author Contributions
FL performed the main experiments and wrote the manuscript. QL, CY, YZ, YW, HM, YQ, YJ, and JM provided laboratory assistance. JM took part in most supplemental experiments and revised the manuscript. YC designed the experiments. LC supervised the study and revised the manuscript.
Funding
This study was supported by the National Institutes of Health grants (R01AI099611 and U19AI089672) and by the National Natural Science Foundation of China (No. 81429004). The Pbs21 mAb clone 13.1 was a gift from Dr. Hiroyuki Matsuoka.
Conflict of Interest
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Supplementary Material
The Supplementary Material for this article can be found online at: https://www.frontiersin.org/articles/10.3389/fmicb.2019.02193/full#supplementary-material
Footnotes
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Keywords: Plasmodium berghei, yeast protein expression, gamete, gamete interaction, transmission-blocking
Citation: Liu F, Liu Q, Yu C, Zhao Y, Wu Y, Min H, Qiu Y, Jin Y, Miao J, Cui L and Cao Y (2019) An MFS-Domain Protein Pb115 Plays a Critical Role in Gamete Fertilization of the Malaria Parasite Plasmodium berghei. Front. Microbiol. 10:2193. doi: 10.3389/fmicb.2019.02193
Received: 31 March 2019; Accepted: 06 September 2019;
Published: 20 September 2019.
Edited by:
Isabelle Morlais, Institut de Recherche pour le Développement (IRD), FranceReviewed by:
Gabriele Pradel, RWTH Aachen University, GermanyKai Matuschewski, Humboldt University of Berlin, Germany
Sonja Frölich, The University of Adelaide, Australia
Copyright © 2019 Liu, Liu, Yu, Zhao, Wu, Min, Qiu, Jin, Miao, Cui and Cao. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
*Correspondence: Liwang Cui, bGN1aUBoZWFsdGgudXNmLmVkdQ==; Yaming Cao, eW1jYW9AY211LmVkdS5jbg==