AUTHOR=Xu Jize , Yu Xiaodong , Lu Mengzhao , Hu Jiajun , Moodley Odeshnee , Zhang Chunlan , Gong Lei , Li Yu 

TITLE=Phylogenetic Analyses of Some Melanoleuca Species (Agaricales, Tricholomataceae) in Northern China, With Descriptions of Two New Species and the Identification of Seven Species as a First Record

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02167

DOI=10.3389/fmicb.2019.02167

ISSN=1664-302X

ABSTRACT=<p>Two new species (<italic>Melanoleuca galerina</italic> and <italic>M. subgrammopodia</italic>) and seven new recorded species from northern China are described here using morphological and molecular methods. <italic>Melanoleuca galerina</italic> is mainly characterized by its hygrophanous pileus, decurrent lamellae, fibrous stipe and spores with round warts. Key characteristics of <italic>M. subgrammopodia</italic> include its discolored pileus, fibrous stipe and urticiform cystidia. The divergence time of <italic>Melanoleuca</italic> fungi as well as the phylogenetic relationships within this genus were analyzed using DNA sequences of the internal transcribed spacer (ITS) and the nuclear large subunit rDNA (nrLSU) gene fragments. Analyses revealed that morphological identifications and phylogenetic relationships were consistent with the results of divergence time, thereby confirming that <italic>M. galerina</italic> and <italic>M. subgrammopodia</italic> are new species.</p>