AUTHOR=Wang Ying , Zhang Yang , Cui Yijie , Sun Zhijian , Zhou Zutao , Hu Sishun , Li Shaowen , Liu Mei , Meng Xianrong , Xiao Yuncai , Shi Deshi , Bi Dingren , Li Zili 

TITLE=Identification of an Integrase That Responsible for Precise Integration and Excision of Riemerella anatipestifer Genomic Island

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02099

DOI=10.3389/fmicb.2019.02099

ISSN=1664-302X

ABSTRACT=<p><italic>Riemerella anatipestifer</italic> is a Gram-negative, pathogenic bacterium, which is harmful to poultry. However, the genomic islands (GIs) in <italic>R. anatipestifer</italic> are not well-studied. In this study, a 10K genomic island was predicted by the bioinformatics analysis of <italic>R. anatipestifer</italic> ATCC 11845, which is widely found in other <italic>R. anatipestifer</italic> genomes. We had first reported the genomic island integration and excision function in <italic>R. anatipestifer</italic>. We successfully constructed the integration plasmid by using the integrase and 53 bp attP elements. The 10K GI was found integrated at the 53 bp attB located in the Arg-tRNA of the <italic>R. anatipestifer</italic> RA-YM chromosome. We identified an integrase that helped in the precise integration and excision in <italic>R. anatipestifer</italic> and elucidated the molecular mechanism of the 10K genomic island integration and excision. Furthermore, we provided a new method for the gene expression and construction of complementary strain.</p>