AUTHOR=Davenport Emily J. , Neudeck Michelle J. , Matson Paul G. , Bullerjahn George S. , Davis Timothy W. , Wilhelm Steven W. , Denney Maddie K. , Krausfeldt Lauren E. , Stough Joshua M. A. , Meyer Kevin A. , Dick Gregory J. , Johengen Thomas H. , Lindquist Erika , Tringe Susannah G. , McKay Robert Michael L. 

TITLE=Metatranscriptomic Analyses of Diel Metabolic Functions During a Microcystis Bloom in Western Lake Erie (United States)

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.02081

DOI=10.3389/fmicb.2019.02081

ISSN=1664-302X

ABSTRACT=<p>This study examined diel shifts in metabolic functions of <italic>Microcystis</italic> spp. during a 48-h Lagrangian survey of a toxin-producing cyanobacterial bloom in western Lake Erie in the aftermath of the 2014 Toledo Water Crisis. Transcripts mapped to the genomes of recently sequenced lower Great Lakes <italic>Microcystis</italic> isolates showed distinct patterns of gene expression between samples collected across day (10:00 h, 16:00 h) and night (22:00 h, 04:00 h). Daytime transcripts were enriched in functions related to Photosystem II (e.g., <italic>psbA</italic>), nitrogen and phosphate acquisition, cell division (<italic>ftsHZ</italic>), heat shock response (<italic>dnaK</italic>, <italic>groEL</italic>), and uptake of inorganic carbon (<italic>rbc</italic>, <italic>bicA</italic>). Genes transcribed during nighttime included those involved in phycobilisome protein synthesis and Photosystem I core subunits. Hierarchical clustering and principal component analysis (PCA) showed a tightly clustered group of nighttime expressed genes, whereas daytime transcripts were separated from each other over the 48-h duration. Lack of uniform clustering within the daytime transcripts suggested that the partitioning of gene expression in <italic>Microcystis</italic> is dependent on both circadian regulation and physicochemical changes within the environment.</p>