AUTHOR=Lu Yan , Liu Peng , Li Changguo , Sha Miao , Fang Jingquan , Gao Jingwen , Xu Xiaoxi , Matthews Karl R. 

TITLE=Prevalence and Genetic Diversity of Cronobacter Species Isolated From Four Infant Formula Production Factories in China

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01938

DOI=10.3389/fmicb.2019.01938

ISSN=1664-302X

ABSTRACT=<p>The aim of this study was to investigate the prevalence and genotypic characteristics of <italic>Cronobacter</italic> isolated from powdered infant formula (PIF) manufacturing facilities and to identify a potential source of contamination. A total of 42 <italic>Cronobacter</italic> isolates (5%) were detected in 835 environmental samples collected during the surveillance study. These isolates included <italic>C</italic>. <italic>sakazakii</italic> (<italic>n</italic> = 37), <italic>C</italic>. <italic>malonaticus</italic> (<italic>n</italic> = 3), and <italic>C. turicensis</italic> (<italic>n</italic> = 2). The isolates were divided into 14 sequence types (STs) by multi-locus sequence typing (MLST) and 21 pulsotypes (PTs) using pulsed-field gel electrophoresis (PFGE). The dominant <italic>C. sakazakii</italic> sequence types were ST3 (<italic>n</italic> = 12) and ST21 (<italic>n</italic> = 10), followed by ST136 (<italic>n</italic> = 6). The major PTs were PT22 (<italic>n</italic> = 12) and PT17 (<italic>n</italic> = 4) based on 100% similarity. Strains isolated from samples collected at the same production facility showed closer phylogenetic relation than those collected from distinct facilities. The result of extensive traceback sampling showed that PIF residues (PIF dust in production areas), fluid beds, drying areas, floors, and soil samples collected adjacent to the production facilities were the primary positive areas for <italic>Cronobacter</italic>. The present study outlines an effective approach to determine prevalence and genetic diversity of <italic>Cronobacter</italic> isolates associated with contamination of PIF.</p>