AUTHOR=Bal Antonin , Oriol Guy , Josset Laurence , Generenaz Laurence , Sarkozy Clémentine , Sesques Pierre , Salles Gilles , Morfin Florence , Lina Bruno , Becker Jérémie , Reynier Frédéric , Mallet François , Pachot Alexandre , Cheynet Valérie , Brengel-Pesce Karen , Trouillet-Assant Sophie 

TITLE=Metagenomic Investigation of Torque Teno Mini Virus-SH in Hematological Patients

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01898

DOI=10.3389/fmicb.2019.01898

ISSN=1664-302X

ABSTRACT=<p>A new member of <italic>Anelloviridae</italic>, named torque teno mini virus (TTMV)-SH, was recently identified in the serum of three Hodgkin’s lymphoma patients suggesting that TTMV-SH may be associated with this type of hematological malignancy. We investigated by metagenomic analysis the presence of TTMV-SH-related viruses in plasma samples (<italic>n</italic> = 323) collected from patients with various hematological malignancies (multiple myeloma (MM, <italic>n</italic> = 256), non-Hodgkin’s lymphoma (NHL, <italic>n</italic> = 20), acute myeloid leukemia (<italic>n</italic> = 10)) and from healthy donors (<italic>n</italic> = 37). TTMV-SH-related strains were identified in 24 samples corresponding to four MM and one NHL patients. Phylogenic analysis revealed that the 24 isolates were close to the TTMV-SH strains previously identified, sharing 79.6–86.7% ORF1 nucleotide sequence identity. These results suggest that TTMV-SH-related viruses might be found in hematological diseases other than Hodgkin’s lymphoma. Due to the high genetic variability within <italic>Anelloviridae</italic> species, the association between a particular medical condition and a new genotype should be interpreted with caution.</p>