AUTHOR=Mori Giorgia , Orena Beatrice Silvia , Cultrera Ilenia , Barbieri Giulia , Albertini Alessandra M. , Ranzani Guglielmina Nadia , Carnevali Ileana , Tibiletti Maria Grazia , Pasca Maria Rosalia 

TITLE=Gut Microbiota Analysis in Postoperative Lynch Syndrome Patients

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01746

DOI=10.3389/fmicb.2019.01746

ISSN=1664-302X

ABSTRACT=<p>Lynch syndrome (LS) is a dominantly inherited condition with incomplete penetrance, characterized by high predisposition to colorectal cancer (CRC), endometrial and ovarian cancers, as well as to other tumors. LS is associated with constitutive DNA mismatch repair (MMR) gene defects, and carriers of the same pathogenic variants can show great phenotypic heterogeneity in terms of cancer spectrum. In the last years, human gut microbiota got a foothold among risk factors responsible for the onset and evolution of sporadic CRC, but its possible involvement in the modulation of LS patients’ phenotype still needs to be investigated. In this pilot study, we performed 16S rRNA gene sequencing of bacterial DNA extracted from fecal samples of 10 postoperative LS female patients who had developed colonic lesions (L-CRC) or gynecological cancers (L-GC). Our preliminary data show no differences between microbial communities of L-CRC and L-GC patients, but they plant the seed of the possible existence of a fecal microbiota pattern associated with LS genetic background, with <italic>Faecalibacterium prausnitzii</italic>, <italic>Parabacteroides distasonis</italic>, <italic>Ruminococcus bromii</italic>, <italic>Bacteroides plebeius</italic>, <italic>Bacteroides fragilis</italic> and <italic>Bacteroides uniformis</italic> species being the most significantly over-represented in LS patients (comprising both L-CRC and L-GC groups) compared to healthy subjects.</p>