AUTHOR=Tourneroche Anne , Lami Raphaël , Hubas Cédric , Blanchet Elodie , Vallet Marine , Escoubeyrou Karine , Paris Alain , Prado Soizic 

TITLE=Bacterial–Fungal Interactions in the Kelp Endomicrobiota Drive Autoinducer-2 Quorum Sensing

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01693

DOI=10.3389/fmicb.2019.01693

ISSN=1664-302X

ABSTRACT=<p>Brown macroalgae are an essential component of temperate coastal ecosystems and a growing economic sector. They harbor diverse microbial communities that regulate algal development and health. This algal holobiont is dynamic and achieves equilibrium <italic>via</italic> a complex network of microbial and host interactions. We now report that bacterial and fungal endophytes associated with four brown algae (<italic>Ascophyllum nodosum</italic>, <italic>Pelvetia canaliculata</italic>, <italic>Laminaria digitata</italic>, and <italic>Saccharina latissima)</italic> produce metabolites that interfere with bacterial autoinducer-2 quorum sensing, a signaling system implicated in virulence and host colonization. Additionally, we performed co-culture experiments combined to a metabolomic approach and demonstrated that microbial interactions influence production of metabolites, including metabolites involved in quorum sensing. Collectively, the data highlight autoinducer-2 quorum sensing as a key metabolite in the complex network of interactions within the algal holobiont.</p>