AUTHOR=Kieffer Nicolas , Poirel Laurent , Fournier Claudine , Haltli Brad , Kerr Russel , Nordmann Patrice 

TITLE=Characterization of PAN-1, a Carbapenem-Hydrolyzing Class B β-Lactamase From the Environmental Gram-Negative Pseudobacteriovorax antillogorgiicola

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01673

DOI=10.3389/fmicb.2019.01673

ISSN=1664-302X

ABSTRACT=<p>The gene encoding the metallo-β-lactamase (MβL) PAN-1 was identified in the genome of the environmental Gram-negative species <italic>Pseudobacteriovorax antillogorgiicola</italic>. PAN-1 shares 57% amino-acid identity with the acquired MβL SPM-1, its closest relative. Kinetic parameters performed on purified PAN-1 showed it displayed a hydrolytic activity toward most β-lactams including carbapenems but spared cefepime and aztreonam. These results further highlight that environmental bacterial species may be reservoirs of MβL encoding genes.</p>