AUTHOR=Djokic Vitomir , Akoolo Lavoisier , Primus Shekerah , Schlachter Samantha , Kelly Kathleen , Bhanot Purnima , Parveen Nikhat 

TITLE=Protozoan Parasite Babesia microti Subverts Adaptive Immunity and Enhances Lyme Disease Severity

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01596

DOI=10.3389/fmicb.2019.01596

ISSN=1664-302X

ABSTRACT=<p>Lyme disease is the most prominent tick-borne disease in the United States. Co-infections with the tick-transmitted pathogens <italic>Babesia microti</italic> and <italic>Borrelia burgdorferi</italic> sensu stricto are becoming a serious health problem. <italic>B. burgdorferi</italic> is an extracellular spirochete that causes Lyme disease while <italic>B. microti</italic> is a protozoan that infects erythrocytes and causes babesiosis. Testing of donated blood for <italic>Babesia</italic> species is not currently mandatory due to unavailability of an FDA approved test. Transmission of this protozoan by blood transfusion often results in high morbidity and mortality in recipients. Infection of C3H/HeJ mice with <italic>B. burgdorferi</italic> and <italic>B. microti</italic> individually results in inflammatory Lyme disease and display of human babesiosis-like symptoms, respectively. Here we use this mouse model to provide a detailed investigation of the reciprocal influence of the two pathogens on each other during co-infection. We show that <italic>B. burgdorferi</italic> infection attenuates parasitemia in mice while <italic>B. microti</italic> subverts the splenic immune response, such that a marked decrease in splenic B and T cells, reduction in antibody levels and diminished functional humoral immunity, as determined by spirochete opsonophagocytosis, are observed in co-infected mice compared to only <italic>B. burgdorferi</italic> infected mice. Furthermore, immunosuppression by <italic>B. microti</italic> in co-infected mice showed an association with enhanced Lyme disease manifestations. This study demonstrates the effect of only simultaneous infection by <italic>B. burgdorferi</italic> and <italic>B. microti</italic> on each pathogen, immune response and on disease manifestations with respect to infection by the spirochete and the parasite. In our future studies, we will examine the overall effects of sequential infection by these pathogens on host immune responses and disease outcomes.</p>