AUTHOR=Watzer Björn , Spät Philipp , Neumann Niels , Koch Moritz , Sobotka Roman , Macek Boris , Hennrich Oliver , Forchhammer Karl TITLE=The Signal Transduction Protein PII Controls Ammonium, Nitrate and Urea Uptake in Cyanobacteria JOURNAL=Frontiers in Microbiology VOLUME=10 YEAR=2019 URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01428 DOI=10.3389/fmicb.2019.01428 ISSN=1664-302X ABSTRACT=
PII signal transduction proteins are widely spread among all domains of life where they regulate a multitude of carbon and nitrogen metabolism related processes. Non-diazotrophic cyanobacteria can utilize a high variety of organic and inorganic nitrogen sources. In recent years, several physiological studies indicated an involvement of the cyanobacterial PII protein in regulation of ammonium, nitrate/nitrite, and cyanate uptake. However, direct interaction of PII has not been demonstrated so far. In this study, we used biochemical, molecular genetic and physiological approaches to demonstrate that PII regulates all relevant nitrogen uptake systems in