AUTHOR=Anantharajah Ahalieyah , Tossens Bastien , Olive Nathalie , Kabamba-Mukadi Benoit , Rodriguez-Villalobos Hector , Verroken Alexia 

TITLE=Performance Evaluation of the MBT STAR®-Carba IVD Assay for the Detection of Carbapenemases With MALDI-TOF MS

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01413

DOI=10.3389/fmicb.2019.01413

ISSN=1664-302X

ABSTRACT=<p><bold>Objectives:</bold> The increasing rate of carbapenem resistance in Gram-negative bacteria is a major public health problem and rapid detection is essential for infection management. We evaluated the performances of the MBT STAR<sup>®</sup>-Carba IVD assay (Bruker Daltonics) to detect carbapenemase-producing organisms (CPO) from bacterial colonies and directly from positive blood culture bottles with MALDI-TOF MS.</p><p><bold>Methods:</bold> We analyzed 130 strains with a reduced susceptibility to at least one carbapenem including 109 CPO (6 KPC, 27 NDM, 21 VIM, 1 IMP, 41 OXA-48-like, 8 OXA-23, 2 OXA-24/-40, and 2 OXA-58) and 21 non-CPO. The assay on colonies was performed with all 130 strains while the assay on spiked blood cultures was performed with 45 strains. Samples were prepared with the MBT STAR<sup>®</sup>-CARBA IVD kit and imipenem hydrolysis by the potential carbapenemase was analyzed with the MBT STAR<sup>®</sup>-BL module (Bruker Daltonics) on MALDI-TOF MS.</p><p><bold>Results:</bold> Performed on colonies, the assay detected all carbapenemase-producing Enterobacteriaceae (<italic>n</italic> = 78), <italic>Pseudomonas</italic> spp. (<italic>n</italic> = 19) and <italic>Acinetobacter</italic> spp. (<italic>n</italic> = 12). All 21 tested non-CPO remained negative resulting in sensitivity and specificity of 100%. Performed on positive blood cultures, the assay detected all carbapenemase-producing Enterobacteriaceae (<italic>n</italic> = 23) and <italic>Pseudomonas</italic> spp. (<italic>n</italic> = 4) but missed 9/12 carbapenemase-producing <italic>Acinetobacter</italic> spp. However, a prolonged imipenem-incubation time of the strain pellet improved carbapenemase detection. Non-CPO from positive blood culture bottles remained negative (<italic>n</italic> = 5) with the assay with the exception of one <italic>Klebsiella pneumoniae</italic> isolate.</p><p><bold>Conclusion:</bold> The MBT STAR<sup>®</sup>-Carba IVD assay is a highly reliable method for the detection of carbapenemase activity in Gram-negative bacteria. However, time-consuming sample preparation steps and reagent costs need to be considered before implementation in a routine clinical microbiology laboratory.</p>