AUTHOR=Prasad Priya , Sanyal Kaustuv , Ghosh Santanu K. 

TITLE=Sth1, the Key Subunit of the RSC Chromatin Remodeling Complex, Is Essential in Maintaining Chromosomal Integrity and Mediating High Fidelity Chromosome Segregation in the Human Fungal Pathogen Candida albicans

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01303

DOI=10.3389/fmicb.2019.01303

ISSN=1664-302X

ABSTRACT=<p>Chromatin architecture influences gene expression and makes specialized chromatin domains. Factors including histone variants, histone modifiers and chromatin remodelers that define chromatin architecture impact chromosome related processes in <italic>Candida albicans</italic>. In this context, we sought to investigate the roles of the ATP-dependent chromatin remodeler, Remodel the Structure of Chromatin (RSC) in chromosome segregation of <italic>C. albicans</italic>. Sth1 is the key ATPase component of RSC and has profound roles in different cellular processes in <italic>Saccharomyces cerevisiae</italic>. We demonstrate that <italic>STH1</italic> is an essential gene in <italic>C. albicans.</italic> The depletion of Sth1 induces pseudohyphal cells, abnormal spindle morphology, sensitivity toward anti-mitotic drugs and global cohesion defect suggesting an important role of Sth1 in kinetochore-microtubule related processes in <italic>C. albicans</italic>. Strikingly, Sth1 is required to maintain clustered kinetochores revealing the fact that RSC is required in kinetochore integrity. Taken together, we show that RSC plays an important role in various chromatin-templated processes including chromosome segregation in <italic>C. albicans</italic>.</p>