AUTHOR=Wei Yuqiu , Zhao Xiangwei , Sun Jun , Liu Haijiao 

TITLE=Fast Repetition Rate Fluorometry (FRRF) Derived Phytoplankton Primary Productivity in the Bay of Bengal

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.01164

DOI=10.3389/fmicb.2019.01164

ISSN=1664-302X

ABSTRACT=<p>The approach of fast repetition rate fluorometry (FRRF) requires a conversion factor (Φ<sub>e : C</sub>/<italic>n</italic><sub>PSII</sub>) to derive ecologically-relevant carbon uptake rates (<italic>PP</italic><sub>z,t</sub>). However, the required Φ<sub>e : C</sub>/<italic>n</italic><sub>PSII</sub> is commonly measured by <sup>14</sup>C assimilation and varies greatly across phytoplankton taxonomy and environmental conditions. Consequently, the use of FRRF to estimate gross primary productivity (<italic>GP</italic><sub>z,t</sub>), alone or in combination with other approaches, has been restricted by both inherent conversion and procedural inconsistencies. Within this study, based on a hypothesis that the non-photochemical quenching (NPQ<sub>NSV</sub>) can be used as a proxy for the variability and magnitude of Φ<sub>e : C</sub>/<italic>n</italic><sub>PSII</sub>, we thus proposed an independent field model coupling with the NPQ<sub>NSV</sub>-based Φ<sub>e : C</sub>/<italic>n</italic><sub>PSII</sub> for FRRF-derived carbon, without the need for additional Φ<sub>e : C</sub>/<italic>n</italic><sub>PSII</sub> in the Bay of Bengal (BOB). Therewith, this robust algorithm was verified by the parallel measures of electron transport rates and <sup>14</sup>C-uptake <italic>PP</italic><sub>z,t</sub>. NPQ<sub>NSV</sub> is theoretically caused by the effects of excess irradiance pressure, however, it showed a light and depth-independent response on large spatial scales of the BOB. Trends observed for the maximum quantum efficiency (F<sub>v</sub>/F<sub>m</sub>), the quantum efficiency of energy conversion (<inline-formula><mml:math id="M1" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mtext>F</mml:mtext><mml:mtext>q</mml:mtext><mml:mi>′</mml:mi></mml:msubsup></mml:mrow></mml:math></inline-formula>/<inline-formula><mml:math id="M2" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mtext>F</mml:mtext><mml:mtext>m</mml:mtext><mml:mi>′</mml:mi></mml:msubsup></mml:mrow></mml:math></inline-formula>) and the efficiency of charge separation (<inline-formula><mml:math id="M3" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mtext>F</mml:mtext><mml:mtext>q</mml:mtext><mml:mi>′</mml:mi></mml:msubsup></mml:mrow></mml:math></inline-formula>/<inline-formula><mml:math id="M4" xmlns:mml="http://www.w3.org/1998/Math/MathML"><mml:mrow><mml:msubsup><mml:mtext>F</mml:mtext><mml:mtext>v</mml:mtext><mml:mi>′</mml:mi></mml:msubsup></mml:mrow></mml:math></inline-formula>) were similar and representative, which displayed a relative maximum at the subsurface and were collectively limited by excess irradiance. In particular, most observed values of F<sub>v</sub>/F<sub>m</sub> in the BOB were only about half of the values expected for nutrient replete phytoplankton. FRRF-based estimates of electron transport at PSII (ETR<sub>RCII</sub>) varied significantly, from 0.01 to 8.01 mol e<sup>−</sup> mol RCII<sup>−1</sup> s<sup>−1</sup>, and showed profound responses to depth and irradiance across the BOB, but fitting with the logistic model. N, P, and irradiance are key environmental drivers in explaining the broad-scale variability of photosynthetic parameters. Furthermore, taxonomic shifts and physiological changes may be better predictors of photosynthetic parameters, and facilitate the selection of better adapted species to optimize photosynthetic efficiency under any particular set of ambient light condition.</p>