AUTHOR=Wang Bing , Song Na , Tang Chunlei , Ma Jinbiao , Wang Ning , Sun Yanfei , Kang Zhensheng 

TITLE=PsRPs26, a 40S Ribosomal Protein Subunit, Regulates the Growth and Pathogenicity of Puccinia striiformis f. sp. Tritici

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.00968

DOI=10.3389/fmicb.2019.00968

ISSN=1664-302X

ABSTRACT=<p>Eukaryotic ribosomes are essential for proliferation, differentiation, and cell growth. RPs26 is a ribosomal subunit structural protein involved in the growth and development process. Little is known about the function of <italic>PsRPs26</italic> in pathogenic fungi. In this study, we isolated the RPs26 gene, <italic>PsRPs26</italic>, from <italic>Puccinia striiformis</italic> f. sp. <italic>tritici</italic> (<italic>Pst</italic>). <italic>PsRPs26</italic> contains a eukaryotic-specific Y62–K70 motif and is more than 90% identical with its ortholog gene in other fungi. PsRPs26 was found to be localized in both the nucleus and cytoplasm. Expression of <italic>PsRPs26</italic> increased when wheat seedlings were inoculated with the <italic>Pst</italic> CYR31 isolate. Moreover, knockdown of <italic>PsRPs26</italic> by a host-induced gene silencing system inhibited growth and limited urediospore production in <italic>Pst</italic>. Our discovery that <italic>PsRPs26</italic> may contribute to the pathogenicity of <italic>Pst</italic> and open a new way in the pathogenic function of <italic>PsRPs26</italic> in cereal rust fungi.</p>