AUTHOR=Herkert Patricia F. , Al-Hatmi Abdullah M. S. , de Oliveira Salvador Gabriel L. , Muro Marisol D. , Pinheiro Rosângela L. , Nucci Márcio , Queiroz-Telles Flávio , de Hoog G. Sybren , Meis Jacques F. 

TITLE=Molecular Characterization and Antifungal Susceptibility of Clinical Fusarium Species From Brazil

JOURNAL=Frontiers in Microbiology

VOLUME=10

YEAR=2019

URL=https://www.frontiersin.org/journals/microbiology/articles/10.3389/fmicb.2019.00737

DOI=10.3389/fmicb.2019.00737

ISSN=1664-302X

ABSTRACT=<p><italic>Fusarium</italic> is widely distributed in the environment and is involved with plant and animal diseases. In humans, several species and species complexes (SC) are related to fusariosis, i.e., <italic>F. solani</italic> SC, <italic>F. oxysporum</italic> SC, <italic>F. fujikuroi</italic> SC, <italic>F. dimerum, F. chlamydosporum, F. incarnatum-equiseti</italic>, and <italic>F. sporotrichoides</italic>. We aimed to investigate the susceptibility of <italic>Fusarium</italic> clinical isolates to antifungals and azole fungicides and identify the species. Forty-three clinical <italic>Fusarium</italic> isolates were identified by sequencing translation elongation factor 1-alpha (<italic>TEF1</italic>α) gene. Antifungal susceptibility testing was performed to the antifungals amphotericin B, itraconazole, voriconazole, posaconazole, and isavuconazole, and the azole fungicides difenoconazole, tebuconazole, and propiconazole. The isolates were recovered from patients with median age of 36 years (range 2–78 years) of which 21 were female. Disseminated fusariosis was the most frequent clinical form (<italic>n</italic> = 16, 37.2%) and acute lymphoblastic leukemia (<italic>n</italic> = 7; 16.3%) was the most commonly underlying condition. A few species described in <italic>Fusarium solani</italic> SC have recently been renamed in the genus <italic>Neocosmospora</italic>, but consistent naming is yet not possible. <italic>Fusarium keratoplasticum</italic> FSSC 2 (<italic>n</italic> = 12) was the prevalent species, followed by <italic>F. petroliphilum</italic> FSSC 1 (<italic>n</italic> = 10), <italic>N. gamsii</italic> FSSC 7 (<italic>n</italic> = 5), <italic>N. suttoniana</italic> FSSC 20 (<italic>n</italic> = 3), <italic>F. solani sensu stricto</italic> FSSC 5 (<italic>n</italic> = 2), <italic>Fusarium</italic> sp. FSSC 25 (<italic>n</italic> = 2), <italic>Fusarium</italic> sp. FSSC 35 (<italic>n</italic> = 1), <italic>Fusarium</italic> sp. FSSC18 (<italic>n</italic> = 1), <italic>F. falciforme</italic> FSSC 3+4 (<italic>n</italic> = 1), <italic>F. pseudensiforme</italic> (<italic>n</italic> = 1), and <italic>F. solani</italic> f. <italic>xanthoxyli</italic> (<italic>n</italic> = 1). Amphotericin B had activity against most isolates although MICs ranged from 0.5 to 32 μg mL<sup>-1</sup>. <italic>Fusarium keratoplasticum</italic> showed high MIC values (8–&gt;32 μg mL<sup>-1</sup>) for itraconazole, voriconazole, posaconazole, and isavuconazole. Among agricultural fungicides, difenoconazole had the lowest activity against FSSC with MICs of &gt;32 μg mL<sup>-1</sup> for all isolates.</p>